Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1398
10
1
gregorian
2020
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1
8
1
online
1
fulltext
en
Molecular Study of Occult Hepatitis C Infection among Iranian Hemophilia Patients Treated with Direct-acting Antiviral Agents
Infectious diseases and public health
Infectious diseases and public health
Original article
Original article
<strong>Introduction: </strong>A new pathological form of HCV named as occult HCV infection (OCI) has been recently characterized by the presence of HCV RNA in liver biopsy and/or peripheral blood mononuclear cell specimens (PBMCs) and the absence of detectable circulating HCV RNA in plasma samples. In this study, we investigated the presence of HCV RNA in PBMCs and plasma samples of 100 hemophilia patients with negative serum HCV RNA. <strong>Methods: </strong>One hundred hemophilia participants receiving IFN-free direct-acting antivirals (DAAs) regimens as a treatment of HCV infection participated in this study. PBMCs were separated with Ficoll before RNA extraction. The HCV genotypes of the positive specimens were also analyzed by RT-PCR assay. Finally, data analysis was performed by SPSS software. <strong>Results: </strong>Our data revealed that out of 100 hemophilia patients, three (3%, 95% CI: 0.006-0.085) were positive for OCI, showing a significant association between OCI and genotype3/drug regimens (<em>p</em> = 0.0203). There was no significant increase at ALT and AST levels in patients with OCI. Moreover, a genotype difference was observed between plasma and PBMCs samples of 1% (1/100) of patients. <strong>Conclusion: </strong>Generally, HCV genotyping in PBMCs along with plasma subtyping before beginning the therapy is vital due to the possibility of OCI detection.
Hemophilia, Occult HCV, Direct-acting antivirals (DAAs)
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6
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-123-5&slc_lang=en&sid=1
Elnaz
Agi
10031947532846003172
10031947532846003172
No
Iranian Comprehensive Hemophilia Care Center, Tehran, Iran
Saber
Asghari
10031947532846003173
10031947532846003173
No
Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Ali
Namvar
10031947532846003174
10031947532846003174
No
Iranian Comprehensive Hemophilia Care Center, Tehran, Iran
Niloofar
Khairkhah
10031947532846003175
10031947532846003175
No
Iranian Comprehensive Hemophilia Care Center, Tehran, Iran
Niloofar
Naderi
10031947532846003176
10031947532846003176
No
Iranian Comprehensive Hemophilia Care Center, Tehran, Iran
Ali
Anvar
10031947532846003177
10031947532846003177
No
Iranian Comprehensive Hemophilia Care Center, Tehran, Iran
Alireza
Azizi Saraji
10031947532846003178
10031947532846003178
No
Iranian Comprehensive Hemophilia Care Center, Tehran, Iran
Azam
Bolhassani
azam.bolhassani@yahoo.com
10031947532846003179
0000000173637406
Yes
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran