en Other Other Original article Original article <strong>Introduction: </strong>The application of biosorbents like bacteria, yeast, and algae is a biotechnological method for eliminating heavy metals from the environment. These microorganisms can also be used for the decontamination of heavy metal in food and water. <strong>Methods</strong>: In this study, we investigated the Cadmium (Cd) biosorption in milk by using <em>Saccharomyces cerevisiae</em>. For this purpose, Cd and <em>S. cerevisiae </em>were added to milk, and the bioremoval process was monitored for four days. We evaluated six variables, including exposure time, temperature, <em>S. cerevisiae</em> concentration, viability yeasts, shaking rate, and initial Cd concentration in the bioremoval process.<strong> Results:</strong> The analysis of ANOVA showed that among the above six variables, <em>S. cerevisiae</em> concentration, initial Cd concentration, and exposure time were statistically significantly associated with Cd removal (<em>P</em> values &le;0.05). The highest biosorption (70%) was observed after 4 days with 30&times;10⁸CFU <em>S. cerevisiae</em> in milk containing 80 &mu;g/L of Cd. <strong>Conclusion</strong>: Our findings provided further evidence for <em>S.</em> <em>cerevisiae</em> as a powerful biosorbent for Cd removal from milk and a potentially safe and green tool for providing safe and healthy food supply. Saccharomyces cerevisiae, Cadmium, Bioremoval 29 33 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-221-3&slc_lang=en&sid=1 Ramona Massoud rm8059@yahoo.com 0000000277609899 No Department of Food Science and Technology, Science and Research branch, Islamic Azad University, Tehran, Iran Anoosheh Sharifan a_sharifan2000@yahoo.com 0000000208496472 Yes Department of Food Science and Technology, Science and Research branch, Islamic Azad University, Tehran, Iran Kianoush Khosravi-Darani kiankh@yahoo.com 0000000202696385 No Department of Food Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences GholamHassan Asadi asadi.srb@gmail.com 0000-0003-2419-657 No Department of Food Science and Technology, Science and Research branch, Islamic Azad University, Tehran, Iran.