en Anti-microbial agents, resistance and treatment protocols Anti-microbial agents, resistance and treatment protocols Original article Original article <strong>Introduction: </strong>An increase in the consumption of antibiotics has raised significant concerns over the treatment of <em>Klebsiella pneumonia</em>-infected patients. In this study, the resistance pattern of <em>K. pneumoniae </em>to antibiotics such as imipenem, meropenem, and ertapenem, as well as the frequency of Metallo-beta-lactamase (MBL) genes, namely VIM, IMP, and NDM-1 were investigated. <strong>Methods:</strong> Following the isolation of 200 <em>K. pneumoniae</em> isolates from 650 clinical samples, the antibiotic resistance pattern of these isolates against different antibiotics was evaluated. The isolates resistant to imipenem, meropenem, and ertapenem were identified, and the presence of VIM, IMP, and NDM-1 genes was examined by using PCR methods. <strong>Results:</strong> The <em>K. pneumoniae</em> isolates exhibited different resistance patterns in response to various antibiotics. The frequency of VIM, IMP, and NDM-1 genes showed that 48 strains are resistant to imipenem, meropenem, and ertapenem in which 15.6% was positive for IMP, 2.42% for VIM, and 1.92% positive for NDM-1 gene. The isolates showed the highest antibiotic resistance to ampicillin (97.5%) and the lowest to meropenem (5.5%). <strong>Conclusion: </strong>Considering carbapenem antibiotics such as imipenem, meropenem, and ertapenem which are known to be among the most frequently used antibiotics for the treatment of <em>K. pneumoniae</em> infections and the involvement of MBL genes in this scenario, we aimed to screen and identify MBL genes responsible for the resistance of <em>K. pneumoniae</em> to imipenem, meropenem, and ertapenem.&nbsp; Klebsiella pneumonia, Metallo-beta-lactamase genes, Antibiotic resistance, PCR 34 39 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-204-1&slc_lang=en&sid=1 Marzieh Saremi marz21@gmail.com 0000-0003-3436-1368 No Department of Microbiology, Qom branch, Islamic Azad University, Qom, Leila Saremi saye83@yahoo.com 0000-0003-0219-6927 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran, Fatemeh Feizy 0000-0002-9184-1969 No Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran Somayeh Vafaei 0000-0002-0758-2133 No Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran Ali Lashkari ALI.LASHKARI@UT.AC.IR 0000-0002-6417-6043 No institute of biochemistry and biophysics university of tehran Zohreh Saltanatpour zohre_saltanatpour@yahoo.com 0000-0002-1616-9404 Yes Medical Genetics Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran Razieh Nazari Nazari 0000-0002-8611-0926 No 1Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran