Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
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admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1397
10
1
gregorian
2019
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1
7
1
online
1
fulltext
en
Isolation and Characterization of a Klebsiella pneumoniae Specific Lytic Bacteriophage from a Hospital Waste-water Treatment Plant
Microbial pathogenesis
Microbial pathogenesis
Original article
Original article
<strong>Introduction: </strong>Phage therapy has gained interest as a potential alternative for treatment of infections caused by multidrug-resistant (MDR) pathogens. This study aimed to isolate a lytic bacteriophage with the potential to lyse clinical isolates of <em>Klebsiella pneumoniae.</em> <strong>Methods: </strong>Water samples were collected from a hospital waste-water treatment plant in Tehran. The samples were filtered and mixed with an overnight grown culture of <em>K. pneumoniae. </em>(ATCC 10031) followed by incubation at 37°C overnight. Phage titration, latent period, and burst size measurements were carried out by the double-layer agar method using the <em>K. pneumoniae </em>ATCC strain. The isolated phage w:as char:acterized by transmission electron microscopy (TEM), thermal, pH, and chloroform stability. Susceptibility of <em>Escherichia coli</em>, <em>Acinetobacter baumannii</em>, <em>Pseudomonas aeruginosa,</em> ESBL producing <em>K. pneumoniae </em>and 51 MDR <em>K. pneumoniae </em>isolates was measured by placing 20 µl of the phage suspension (10<sup>8</sup> PFU) onto bacterial lawns followed by incubation at 37°C overnight. Formation of clear zones indicated susceptibility. <strong>Results: </strong>The isolated lytic bacteriophage formed small clear plaques with a latent period of 40 min and a burst time of 52 min, corresponding to 35-40 phage particles per infected cell. TEM results showed that the phage resembled the tailed <em>Siphoviridae</em> family and was designated vB_KpnS-Teh.1. The phage vB_KpnS-Teh.1 was most stable at 37°C, pH 7 and was resistant to chloroform. <strong>Conclusion: </strong>The isolated lytic phage showed specificity towards <em>K. pneumoniae. </em>Further research will determine its potential in the treatment of <em>K. pneumoniae </em>infections.
Klebsiella pneumoniae, Bacteriophage, Waste-water, Siphoviridae
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http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-176-2&slc_lang=en&sid=1
Mahboubeh
Soleimani Sasani
mahbobeh soleimani@yahoo.com