en Epidemiologic studies including microbial genotyping, phenotyping and serotyping Epidemiologic studies including microbial genotyping, phenotyping and serotyping Original article Original article <strong>Introduction: </strong>Rabies is a highly fatal disease. However, it is quite preventable. Community awareness about rabies is one of the key components for prevention, which should be assessed and routinely monitored by standard questionnaires. We aimed to develop and validate a Persian knowledge, attitude and practice questionnaire for rabies (PKAP-Rabies) in the general population of Iran.<strong> Methods: </strong>The questionnaire was developed based on existing literature and conducting focus group discussions with experts in the field. Content, face, and construct validity were checked by gathering the opinion of 10 experts in the field. Test-retest reliability was assessed by re-administrating the questionnaire to the same individuals after a 15-days interval. <strong>Results: </strong>The questionnaire consisted of 64 items, covering five domains including &lsquo;demographics&rsquo; (19 items), knowledge about animal and human rabies (14 and 10 items, respectively), attitude towards rabies prevention in animals (6 items), and practice towards rabies prevention/control (14 items). The questionnaires were mainly filled by young (mean= 28.6, SD= 10.3 years) women (63.3%) with a university-level educational background (36.7%). Most knowledge questions had a proper difficulty level (average difficulty index= 20-80%). Wilcoxon test also showed proper test-retest reliability for this questionnaire (P_Wilcoxon > 0.05). <strong>Conclusions: </strong>The PKAP-Rabies questionnaire appeared to be feasible, valid and reliable for assessing KAP towards rabies in the general population of Iran with potential application in future large-scale surveys. Information from such surveys can provide insight into adopting prevention and control measures, and would allow us to evaluate the impact of current and upcoming interventions. Rabies, Knowledge, Attitude, Practice, Iran 67 71 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-121-4&slc_lang=en&sid=1 Maryam Fazeli m_fazeli@pasteur.ac.ir 0000-0003-4048-4778 No National Collaborating Center for References and Research on Rabies, Virology Department, Pasteur Institute of Iran, Tehran, Iran Mahsa Golahdouz mahsa.ko73@yahoo.com No National Collaborating Center for References and Research on Rabies, Virology Department, Pasteur Institute of Iran, Tehran, Iran Rouzbeh Bashar dr.r.bashar@gmail.com 0000-0001-5469-4472 No National Collaborating Center for References and Research on Rabies, Virology Department, Pasteur Institute of Iran, Tehran, Iran Masoumeh Arab Baferani a_masoumeh@yahoo.com 0000-0001-5803-2847 No National Collaborating Center for References and Research on Rabies, Virology Department, Pasteur Institute of Iran, Tehran, Iran Amin Doosti Irani doostiiraniamin@gmail.com 0000-0003-0623-7503 No Department of Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran Behzad Pourhossein pourhossein.b@gmail.com 0000-0002-4263-0612 No Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Sana Eybpoosh s_eybpoosh@pasteur.ac.ir 0000-0002-2304-8352 Yes Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging infectious diseases, Pasteur Institute of Iran, Tehran, Iran