Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61882/JoMMID
14
8888
13
en
jalali
1398
7
1
gregorian
2019
10
1
7
4
online
1
fulltext
en
Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<strong>Introduction: </strong><em>Pseudomonas</em> <em>aeruginosa</em> is an opportunistic pathogen of clinical importance, particularly in immunocompromised and burn patients. This bacterium is becoming resistant to many antibiotics via intrinsic or acquired mechanisms. Mutations in anti-mutator genes, such as <em>pfpI</em>, can be a potential intrinsic mechanism of antibiotic resistance. This study aimed to evaluate the possible effects of mutations of this gene on coding proteins of multi-drug resistant <em>P. aeruginosa</em> isolates. <strong>Methods: </strong>The antibiotic resistance pattern of 50 <em>P. aeruginosa</em> isolates against 9 anti-<em>pseudomonas</em> antibiotics was determined by the disk diffusion method. PCR, followed by sequencing, detected the mutations in the <em>pfpI</em> gene. The retrieved sequences were translated to the corresponding amino acid sequences using an online protein database. The amino acid sequences in mutated isolates were compared with the reference sequence using a multiple alignment method. <strong>Results: </strong>Out of 50 isolates, 43 (86%) were resistant to all antibiotics. Sequencing and multiple alignment analyses showed that amino acids in positions 21, 24, and 57 of <em>pfpI</em> gene were changed in resistant isolates, and all these mutations were observed in each isolate. Homology modeling showed that these amino acids were part of a cleft on the protease. The other point mutations resulted in amino acid changes were in positions 67, 83, and 165. <strong>Conclusion: </strong>The data obtained in this study showed that the <em>pfpI</em> gene of <em>P. aeruginosa</em> might have a significant effect on response to antibiotics. Further epidemiologic and comprehensive studies are required to confirm these findings.
Burns, Pseudomonas aeruginosa, Drug Resistance, Amino Acid Sequence, Point Mutation
127
131
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-129-1&slc_lang=en&sid=1
Maryam
Khalili-Samani
maryamkhalili1983@hotmail.com