Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1397
1
1
gregorian
2018
4
1
6
2
online
1
fulltext
en
Single Nucleotide Polymorphism of the Interferon-γ Gene (IFN-γ +874 T/A) and the Prognosis of Hepatitis B Infection
Host-pathogen interactions and susceptibility factors
Host-pathogen interactions and susceptibility factors
Original article
Original article
<strong>Introduction: </strong>Chronic Hepatitis B virus infection is a multifactorial disease with a variety of clinical outcomes. Since interferon-gamma (IFN-γ) is a significant immune factor in antiviral defense, this case-control study aimed to investigate the potential relationship between single nucleotide polymorphism of rs2430561 and hepatitis B infection outcome in a population of Birjand city, eastern Iran. <strong>Methods: </strong>Blood samples were collected from 60 chronically HBV- infected patients and 60 healthy subjects with the history of HBV infection. Genomic DNA was extracted from whole blood by the salting-out method. The first intron of IFN-γ with a length of 264 bp was amplified by Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) followed by sequencing. <strong>Results: </strong>Our results exhibited a statistically significant difference between patients and control individuals (<em>p</em>-value<0.001). The frequency of the allele A was 73.3% in HBV- infected patients, whereas in controls (individuals with a history of HBV infection) it was 46.7%. <strong>Conclusion:</strong> A statistically significant relationship was found between the IFN-γ (+874T/A, rs2430561) single nucleotide polymorphism (SNP) and chronic HBV infec­tion in the studied population. The obtained results showed that HBV infected individuals with T allele have less risk of progressing to chronic HBV infection. It also suggests that the homozygous carriers of the A allele are more vulnerable to chronic HBV infection.
Chronic Hepatitis B, Single Nucleotide Polymorphism, Interferon-γ (+874T/A), Iran
43
47
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-156-1&slc_lang=en&sid=1
Mohammad Sadegh
Naghizadeh
msnaghizadeh92@gmail.com
10031947532846002417
10031947532846002417
No
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
Mohsen
Naseri
naseri_m2003@yahoo.com
10031947532846002418
10031947532846002418
No
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
Mohammad
Fereyduni
dr.m.fereidouni@gmail.com
10031947532846002419
10031947532846002419
No
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
Masoud
Ziaee
dr.m.ziaee@gmail.com
10031947532846002420
10031947532846002420
No
3Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
Abdolghader
Tane
ghadertane94@gmail.com
10031947532846002421
10031947532846002421
No
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
Hamidreza
Safari
hamidrezasafari941@gmail.com
10031947532846002422
10031947532846002422
No
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
Neda
Mahavar
neda.mahavar91@gmail.com
10031947532846002423
10031947532846002423
No
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
Roya
Mahdavi
royamahdavi1986@gmail.com
10031947532846002424
10031947532846002424
No
4Department of Immunology, Birjand University of Medical Sciences, Birjand, Iran
Gholamreza
Anani Sarab
ghansa@yahoo.com
10031947532846002425
10031947532846002425
Yes
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran