Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1396
4
1
gregorian
2017
7
1
5
3
online
1
fulltext
en
Molecular Detection of Antibiotic Resistance Determinants in Lactobacillus Bacteria Isolated from Human Dental Plaques
Anti-microbial agents, resistance and treatment protocols
Anti-microbial agents, resistance and treatment protocols
Original article
Original article
<strong>Introduction: </strong>Dental caries is a significant public health problem which destroys the calcified tissues of the teeth. The frequency of<em> lactobacilli </em>in dental caries is usually high. The objective of this study was to evaluate the incidence of these bacteria in dental caries and to identify the antibiotic resistance patterns of them. <strong>Methods: </strong>Specimens from dental plaque were collected from 30 patients. Phenotypic (Gram staining, cell morphology, and biochemical tests) and molecular (PCR) methods were used for identification of <em>Lactobacillus</em> bacteria. The antimicrobial sensitivity test was performed by standard Kirby-Bauer disk diffusion method to determine their resistance to chloramphenicol, penicillin, vancomycin, nitrofurantoin, cefixime, cefazolin, amoxicillin-clavulanic acid, and tetracycline. Antibiotic resistance genes were detected using multiplex PCR method as well. <strong>Results: </strong>Fourteen out of 30 (46/6%) samples were <em>Lactobacillus</em> positive. The <em>mecA</em> was the essential gene responsible for penicillin resistance. Also, the <em>bla<sub>TEM</sub></em> and bla<em><sub>OXA-</sub></em><sub>1 </sub>genes had more significant role in cefazolin and cefixime resistance than <em>bla<sub>SHV</sub></em>, and <em>these </em>two genes had the similar role in the amoxicillin-clavulanic acid resistance. Also, tetracycline resistance was caused by both <em>tetK</em> and <em>tetM</em> genes identically. <strong>Conclusion: </strong>In conclusion, <em>Lactobacillus </em>bacteria had an essential role in the formation of dental plaque and showed multidrug-resistant patterns to commonly used antibiotics.
Dental plaque, Lactobacillus, Multiplex PCR, resistance genes
51
55
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-116-2&slc_lang=en&sid=1
Sahar
Nouri Gharajalar
saharnouri@yahoo.com
10031947532846001994
10031947532846001994
Yes
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Masoumeh
Firouzamandi
10031947532846001995
10031947532846001995
No
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran