Journal of Medical Microbiology and Infectious Diseases
Journal of Medical Microbiology and Infectious Diseases
JoMMID
Medical Sciences
http://jommid.pasteur.ac.ir
1
admin
2345-5349
2345-5330
8
10.61186/JoMMID
14
8888
13
en
jalali
1395
4
1
gregorian
2016
7
1
4
3
online
1
fulltext
en
In Vitro Ovicidal Activity of Nematophagous Fungus Paecilomyces lilacinus on the Eggs of Parasitic Helminths
Other
Other
Original article
Original article
<div dir="ltr"><strong><span style="font-family:garamond,serif;"><span style="font-size:10.0pt;">Introduction: </span></span></strong><span style="font-family:garamond,serif;"><span style="font-size:10.0pt;">The nematophagus fungi have been suggested as an alternative way to eliminate the zoonotic helminths eggs from the environment. In the present study, we evaluated the ovicidal activity of a fungus, <em>Paecilomyces lilacinus</em>, recovered from a compost soil, on the eggs of parasitic helminths under <em>in vitro</em> condition. <strong>Methods: </strong>Water suspension of the soil samples collected from different areas of Iran were transferred to 2% water-agar culture media baited with <em>Rhabditis </em>sp. larvae. The larvae-infecting fungi were harvested on potato dextrose agar (PDA) media. The sequencing of a beta-tubulin gene identified the nematophagus fungi as <em>P. lilacinus</em>. Eggs of three helminth species, <em>Syphacia obvelata, Hymenolepis diminuta</em> and<em> Echinococcus granulosus </em>were exposed to one isolate of the recovered fungi, and the ovicidal activity was monitored for up to 21 days. <strong>Results:</strong> Out of 300 samples, only three compost soils, contained the nematophagus fungus<em> P. lilacinus</em>. Microscopical examinations revealed invasion of the helminths eggs by the <em>P. lilacinus</em>. The eggs of<em> H. diminuta</em> were more vulnerable to this fungus invasion while <em>E. granulosus </em>eggs were the least affected ones (<em>P</em><0.05). <strong>Conclusion: </strong><em>Paecilomyces lilacinus</em> can grow on fresh feces and attack the eggs of the parasitic helminths. Therefore, a combination of its hyphae with feces or administration of the spores in the animals’ food may reduce the helminths eggs in the environment and consequently intervene with the transmission of parasitic helminthes in the animal houses.</span></span></div>
Nematophagous, Paecilomyces lilacinus, Syphacia obvelata, Hymenolepis diminuta, Echinococcus granulosus
52
56
http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-140-1&slc_lang=en&sid=1
Faezeh
Najafi
faezeh_najafi@yahoo.com
10031947532846001673
10031947532846001673
No
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Saied Reza
Naddaf
snaddaf_2001@yahoo.com
10031947532846001674
10031947532846001674
No
Department of Parasitology, Research Center for Emerging and Reemerging Diseases Pasteur Institute of Iran, Tehran, Iran
Sasan
Rezaie
srezaie@tums.ac.ir
10031947532846001675
10031947532846001675
No
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Eshrat Beigom
Kia
molaviva@tums.ac.ir
10031947532846001676
10031947532846001676
No
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Gholamreza
Mowlavi
molaviva@tums.ac.ir
10031947532846001677
10031947532846001677
Yes
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran