en Infectious diseases and public health Infectious diseases and public health Original article Original article <div dir="ltr"><strong><span style="font-family:garamond,serif;"><span style="font-size:10.0pt;">Introduction: </span></span></strong><span style="font-family:garamond,serif;"><span style="font-size:10.0pt;">The human immunodeficiency virus (HIV) is a threat to global health and the need for finding new methods of antivirus research, in particular against HIV has increased in recent years. In this study, we investigated the ability of the cold atmospheric pressure plasma jet (CAPPJ) using helium to inhibit the replication of HIV virions. <strong>Methods: </strong>Single cycle replicable HIV (SCR-HIV) virions were produced by transfection of HEK293T cells with pmzNL4-3, pSPAX.2, and pMD2.G plasmids. The HeLa immortal cell line was infected with the virus SCR-HIV and then irradiated by CAPPJ at different voltages and times. The amount of the P24 antigen inside cell supernatant was measured by an ELISA method. Moreover, the cytotoxicity of this plasma jet and the viability of cells were evaluated by the XTT method. <strong>Results: </strong>The inhibition of HIV by CAPPJ increased with increasing of voltage and the time of radiation. The highest voltage of 12 kv at 240 s caused virus inhibition; however, the cytotoxicity of HeLa cell line also elevated with increasing of voltage and time. <strong>Conclusion: </strong>CAPPJ substantially suppresses HIV infection <em>in vitro </em>while causing toxicity for HeLa cell line</span></span></div> Cold atmospheric pressure plasma jet, Human Immunodeficiency Virus, Single cycle replicable virions, Plasma jet 62 67 http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-135-1&slc_lang=en&sid=1 Mohammad Reza Amiran mohammadr.amiran@gmail.com No Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran. Abbas Akhavan Sepahi akhavansepahy@gmail.com No Department of Microbiology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran. Rezvan Zabiollahi rezvanzabihollahi@gmail.com No Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran. Hamid Ghomi h-gmdashty@sbu.ac.ir No Laser and Plasma Research Institute, Shahid Beheshti University, Evin, 1983963113 Tehran, Iran. Seyed Bahman Momen sbmomen@gmail.com No Department of Pilot Biotechnology, Pasteur Institute of Iran, Tehran, Iran. Mohammad Reza Aghasadeghi mr_sadeqi@yahoo.com Yes Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.