TY - JOUR JF - JoMMID JO - JoMMID VL - 9 IS - 3 PY - 2021 Y1 - 2021/9/01 TI - Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine TT - N2 - Introduction: Extended-Spectrum β-Lactamases (ESBLs) hydrolyze broad-spectrum cephalosporin, monobactam, and penicillin. This study investigated ESBL-producing Escherichia coli and Klebsiella pneumoniae bacteria in the Gaza strip and explored their susceptibility to various antimicrobials to provide a reference for physicians in managing the hospital infection. Methods: Ninety-six isolates, comprising 69 E. coli and 27 K. pneumoniae were obtained from urine, wound, blood, and ear discharge samples from April-June 2013 in Gaza hospitals. The ESBL-producing isolates were screened using the double-disc diffusion test. Antibiotics susceptibility test was determined by the disc diffusion method on Mueller-Hinton agar, and PCR identified β-lactamases genes. Results: Our results revealed high rates of ESBL-producing K. pneumoniae (59.3%) and E. coli (39.1%) among isolates. About 65.1% of ESBL-producing isolates were susceptible to imipenem while exhibited 100% resistance to cefotaxime and ampicillin and 74.4% to sulfamethoxazole/trimethoprim. Except for imipenem, higher antibiotic resistance rates were observed among ESBL producers than non-ESBL producers. This study showed that the antimicrobial resistance and ESBLs were higher in K. pneumoniae isolates than E. coli isolates, and most K. pneumoniae isolates harbored simultaneously two or three β-lactamases-encoding genes. Conclusion: High ESBL-producing rates among K. pneumoniae and E. coli isolates and higher resistance rates to antibiotics among ESBL compared to non-ESBL producing isolates necessitate antimicrobial resistance surveillance and molecular characterization of ESBLs-producing bacteria to achieve a specific treatment. SP - 133 EP - 141 AU - Tayh, Ghassan AU - Al Laham, Nahed AU - Fhoula, Imene AU - Abedelateef, Nahed AU - El-Laham, Mohamed AU - Elkader Elottol, Abed AU - Ben Slama, Karim AD - Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisie KW - Beta-lactamase genes KW - ESBL isolates KW - Antibiotics resistance KW - Carbapenem KW - E. coli KW - K. pneumonia UR - http://jommid.pasteur.ac.ir/article-1-362-en.html DO - 10.52547/JoMMID.9.3.133 ER -