AU - Naddaf, Saied Reza AU - Mohabati, Reyhaneh AU - Vahabpor, Rouhollah AU - Naeimi, Sabah AU - Eybpoosh, Sana TI - Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR PT - JOURNAL ARTICLE TA - JoMMID JN - JoMMID VO - 8 VI - 3 IP - 3 4099 - http://jommid.pasteur.ac.ir/article-1-267-en.html 4100 - http://jommid.pasteur.ac.ir/article-1-267-en.pdf SO - JoMMID 3 AB  - Introduction: Leptospirosis is a significant public health problem in the Caspian littoral of Iran comprising Gilan, Mazandaran, and Golestan provinces. In Golestan province, serology assays indicated anti-Leptospira antibodies in animals and humans; however, no reliable record of infections in patients with signs and symptoms of the disease is available. Methods: We employed the indirect immunofluorescent antibody assay (IFA), and two PCR assays, a real-time PCR (qPCR) and a nested-PCR targeting 16S rDNA (rrs) sequence for diagnosis of leptospirosis in febrile patients in Golestan province. Results: Out of 52 febrile patients, 25 (48.07%) had antibody titers ≥1/80 by IFA, and were defined as positive. In 7, 9, and 7 individuals, the antibody titers were 1/40, 1/20, and 1/10, respectively, and 4 had no antibodies. The qPCR and nested PCR detected leptospiral DNA in 55.75% and 67.3% of the patients' sera, respectively. The two PCR assays had a Kappa agreement of 0.53 (P< 0.0001), suggesting a moderate agreement, and showed a significant reverse association with the IFA titers (P<0.05). Conclusion: Our results suggest that higher antibody titers accompanied the spirochete's removal from blood by the patient's immune response. Hence, a reliable diagnosis of Leptospira infection necessitates deploying a DNA-based method alongside serology. CP - IRAN IN - Jomhori St., 12th Farvardin Ave. #69 LG - eng PB - JoMMID PG - 98 PT - Original article YR - 2020