%0 Journal Article %A Naghizadeh, Mohammad Sadegh %A Naseri, Mohsen %A Fereyduni, Mohammad %A Ziaee, Masoud %A Tane, Abdolghader %A Safari, Hamidreza %A Mahavar, Neda %A Mahdavi, Roya %A Anani Sarab, Gholamreza %T Single Nucleotide Polymorphism of the Interferon-γ Gene (IFN-γ +874 T/A) and the Prognosis of Hepatitis B Infection %J Journal of Medical Microbiology and Infectious Diseases %V 6 %N 2 %U http://jommid.pasteur.ac.ir/article-1-149-en.html %R 10.29252/JoMMID.6.2.3.43 %D 2018 %K Chronic Hepatitis B, Single Nucleotide Polymorphism, Interferon-γ (+874T/A), Iran, %X Introduction: Chronic Hepatitis B virus infection is a multifactorial disease with a variety of clinical outcomes. Since interferon-gamma (IFN-γ) is a significant immune factor in antiviral defense, this case-control study aimed to investigate the potential relationship between single nucleotide polymorphism of rs2430561 and hepatitis B infection outcome in a population of Birjand city, eastern Iran. Methods: Blood samples were collected from 60 chronically HBV- infected patients and 60 healthy subjects with the history of HBV infection. Genomic DNA was extracted from whole blood by the salting-out method. The first intron of IFN-γ with a length of 264 bp was amplified by Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) followed by sequencing. Results: Our results exhibited a statistically significant difference between patients and control individuals (p-value<0.001). The frequency of the allele A was 73.3% in HBV- infected patients, whereas in controls (individuals with a history of HBV infection) it was 46.7%. Conclusion: A statistically significant relationship was found between the IFN-γ (+874T/A, rs2430561) single nucleotide polymorphism (SNP) and chronic HBV infec­tion in the studied population. The obtained results showed that HBV infected individuals with T allele have less risk of progressing to chronic HBV infection. It also suggests that the homozygous carriers of the A allele are more vulnerable to chronic HBV infection. %> http://jommid.pasteur.ac.ir/article-1-149-en.pdf %P 43-47 %& 43 %! IFN-γ polymorphism effect on HBV clearance %9 Original article %L A-10-156-1 %+ Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran %G eng %@ 2345-5349 %[ 2018