Pasteur Institute of Iran

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Introduction: Hepatitis A virus (HAV) infection poses a significant public health problem worldwide, especially in developing countries. This study investigated the effect of vaccination policies on the HAV seropositivity of Syrian immigrants and local Turkish people. Methods: The anti-HAV antibodies of 6007 patients, including 5613 (93.4%) Turks and 394 (6.6%) Syrian suspected of HAV infection, were analyzed by the chemiluminescent microparticle immunoassay (CMIA) method. Results: In our study, total anti-HAV positivity was higher in Turkish patients than in Syrian patients in the 0-9 age group, while in the 10-19, 20-29, and 30-39 age groups, the rate was higher in Syrians. Anti-HAV seropositivity was significantly higher in Turkish male patients than female patients. The young adult and adult age groups of Turks were more at risk of HAV infection, i.e., when the disease is symptomatic. Conclusion: Vaccination of young and young adult seronegative Turks and ensuring Syrian children's participation in the routine vaccination program implemented in our country is a requirement for preventing HAV infection.

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Introduction: Early detection of acute Hepatitis A virus infection (HAV) allows adopting proper treatment measures, rapid recovery, and avoiding side effects. This study compares PCR assay with serology for diagnosing acute HAV infection. Methods: Twenty samples from patients presenting clinical symptoms of acute hepatitis were tested for anti-HAV IgM antibodies. Genomic RNA was extracted from IgM-positive samples, cDNA was synthesized and examined for genomic HAV using a specific HAV real-time detection kit and a nested PCR. Results: Among 20 sera, 14 were positive for anti-HAV IgM antibodies. The specific real-time PCR and nested PCR showed agreement, and both detected HAV genetic material in 3 out of 14 samples. Conclusion: High levels of anti-HAV IgM antibodies do not necessarily indicate acute HAV infection in people presenting clinical symptoms of the disease.  Measuring IgM antibody levels alongside molecular detection of virus genome by DNA-based methods assay can lead to an accurate, timely, and reliable diagnosis of active HAV infection.