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:: Volume 3, Issue 1 And 2 (1-2015) ::
JoMMID 2015, 3(1 And 2): 29-34 Back to browse issues page
Genetic Characterization of Salmonella Typhimurium Isolates from Faeces of Children with Gastroenteritis Hospitalized in Baqiatollah-Azam Hospital, Tehran, Iran
Hamid Staji , Alfreda Tonelli , Taghi Zahraei Salehi , Mariangela Iorio , Federica Lopes
Assistant professor Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Abstract:   (2493 Views)

Introduction: In Iran, invasive nontyphoidal Salmonella (iNTS) disease causes severe bacteremic illness among children <5 years old. The global yearly incidence of iNTS disease in children was reported to be in the 3.4 (range  2.1-6.5) million cases,  (overall incidence 49 cases (range 30-94) per 100,000 population), the iNTS case-fatality ratio (CFR) of 20% yielded 681,316  deaths annually. Methods: The microarray analysis enables identification of the strains that have the 90kb Salmonella typhimurium virulence plasmid, presence or absence of the Salmonella pathogenicity islands (SPIs), adherence factors and other  virulence determinants. Twelve isolates of S. typhimurium obtained from faeces of children with gastroenteritis were analyzed  by microarray technique. Results: The virulence plasmid was present in 83.33% of isolates and all the isolates contained the  SPI-4 and SPI-5. None of the strains had the cytolethal distending toxin, cdtB. All strains were positive for rck and mig-14. The adherence genes were present in all the strains in the range of  51.55%  to 73.20% of the adherence genes interrogated in the  microarray. Two strains were the least pathogenic S. typhimurium. Conclusion: Microarray analysis proved to be a valuable tool in confirmation of serotyping results and genetic characterization of S. Typhimurium.

Keywords: Salmonella typhimurium, Gastroenteritis, Microarray Analysis
Full-Text [PDF 427 kb]   (954 Downloads)    
Type of Study: Original article | Subject: Epidemiologic studies including microbial genotyping, phenotyping and serotyping
Received: 2016/07/1 | Accepted: 2016/11/13 | Published: 2016/12/19
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Staji H, Tonelli A, Zahraei Salehi T, Iorio M, Lopes F. Genetic Characterization of Salmonella Typhimurium Isolates from Faeces of Children with Gastroenteritis Hospitalized in Baqiatollah-Azam Hospital, Tehran, Iran. JoMMID . 2015; 3 (1 and 2) :29-34
URL: http://jommid.pasteur.ac.ir/article-1-97-en.html


Volume 3, Issue 1 And 2 (1-2015) Back to browse issues page
Journal of Medical Microbiology and Infectious Diseases
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