Volume 3, Issue 3 And 4 (7-2015)                   JoMMID 2015, 3(3 And 4): 67-70 | Back to browse issues page

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Abbasi S, Zamanzad B. Detection of icaABCD Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus from Patients in Iran. JoMMID. 2015; 3 (3 and 4) :67-70
URL: http://jommid.pasteur.ac.ir/article-1-91-en.html
Department of Microbiology and Immunology, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract:   (4102 Views)

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that causes several nosocomial or community-acquired infections. Adhesion to surfaces and subsequent biofilm formation are the major phases of a staphylococcal infection. The aim of this study was to detect the presence of icaABCD genes in clinical isolates of MRSA. Methods: A total of 110 clinical Staphylococcus aureus isolates were collected from two teaching hospitals in Shahrekord (Hajar and Kashani hospitals). The MRSA isolates were detected by an antibiotic susceptibility test. A microtiter tissue plate assay was used to detect the phenotypic biofilm formation. A polymerase chain reaction (PCR) was performed to detect the presence of icaABCD genes. Results: The microtiter plate assay results showed that attachment abilities were strong in 26 (23.6%) strains, moderate in 30 (27.2%) strains, and weak in 16 (14.54%) strains. The prevalence of the icaA, icaB, icaC, and icaD genes among the studied isolates were as follows: 42 isolates were icaA positive (38.18%), 34 icaB positive (30.9%), 46 icaC positive (41.8%), and 50 were icaD positive (45.4%). Conclusion: The high prevalence of icaA/D harboring S. aureus among the clinical isolates suggests that the risk of persistent infections in the hospital settings is considerably high.

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Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2016/03/29 | Accepted: 2017/04/11 | Published: 2017/05/22

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