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:: Volume 2, Issue 4 (10-2014) ::
JoMMID 2014, 2(4): 163-166 Back to browse issues page
Detection of Carbapenemases Emerging in Acinetobacter baumannii Clinical Isolates by Modified Hodge Test
Mojtaba Moosavian , Nasim Shams , Mehrandokht Sirous
student Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abstract:   (2146 Views)

Introduction: Acinetobacter baumannii, a known causative agent of nosocomial infections, is one of the highly antibiotic-resistant gram-negative bacilli. Carbapenem-resistant Acinetobacter isolates are increasingly reported worldwide. Carbapenems such as imipenem and meropenem are efficient antimicrobial agents commonly used for the treatment of infections caused by multi- resistant A. baumannii strains. Some reports indicate treatment failure due to antibiotic resistant A. baumannii strains. The aim of this study was to determine antibiotic resistance pattern and prevalence carbapenemase production in A. baumannii isolates. Method: A total of 100 A. baumannii isolates were identified from clinical specimens by standard chemical tests. The samples were collected from the patients hospitalized in two teaching hospitals of Ahvaz, southwestern of Iran. The susceptibility of isolates to different antibiotics was determined by the disk diffusion method based on Clinical Laboratory Standards Institute (CLSI) direction. The Modified Hodge Test (MHT) was performed for detection of carbapenemase - producing A. baumannii isolates. Results: The isolates showed the highest resistance to ciprofloxacin (98%). The resistance rate to cefotaxime, ceftazidime, and piperacillin was 97%, gentamicin, amikacin, and meropenem 96%, imipenem 95%, cefepime 93%, and tetracycline 60%. Most of the isolates (99%) were sensitive to colistin. Among the100 A. baumannii isolates, 53 (53%) were positive for carbapenemase production by MHT. Conclusion: This study emphasizes dissemination of carbapenem resistant A. baumannii strains. Our study also showed that the MHT has an excellent sensitivity for detecting carbapenemase - producing A. baumannii isolates.

Keywords: Acinetobacter baumannii, Antibiotic Resistance, Carbapenemase, Modified Hodge Test
Full-Text [PDF 334 kb]   (1223 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2016/03/15 | Accepted: 2016/06/28 | Published: 2016/08/2
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Moosavian M, Shams N, Sirous M. Detection of Carbapenemases Emerging in Acinetobacter baumannii Clinical Isolates by Modified Hodge Test. JoMMID . 2014; 2 (4) :163-166
URL: http://jommid.pasteur.ac.ir/article-1-89-en.html


Volume 2, Issue 4 (10-2014) Back to browse issues page
Journal of Medical Microbiology and Infectious Diseases
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