Volume 10, Issue 3 (9-2022)                   JoMMID 2022, 10(3): 135-140 | Back to browse issues page

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Soubeiga S T, Charlotte K, Zoure A A, Compaoré T R, Zida S, Kagembega A, et al et al et al . SARS-CoV-2 Variants Screening in Burkina Faso. JoMMID 2022; 10 (3) :135-140
URL: http://jommid.pasteur.ac.ir/article-1-483-en.html
SARS-CoV-2 Variants Screening in Burkina Faso
Serge Theophile Soubeiga , Kinda Charlotte , Abdou Azaque Zoure , Tegwinde Rebeca Compaoré , Sylvie Zida , Alidou Kagembega , Tani Sagna , Charlemagne Dabiré , Oumarou Ouedraogo , Nadiyah Lasisi , Alexia Kabore , Farida Milène Zida , Binta Fofana , Stéphane Somé , Abdoul Nikiema , Dinanibè Kambire , Dramane Zongo , Issiaka Soulama , Charles Sawadogo , Sandrine Gampini , Henri Gautier Ouedraogo
Institut de Recherche en Sciences de la Santé (IRSS) /Laboratoire de Recherche Biomédicale (LaReBio), Ouagadougou, Burkina Faso
Abstract:   (1165 Views)
Introduction: In Sub-Saharan Africa, the data on the mutations and variants of circulating SARS-CoV-2 is limited. This study aimed to screen specific mutations and variants of SARS-CoV-2 circulating in Burkina Faso. Methods: This study included symptomatic and asymptomatic individuals who underwent diagnostic testing for SARS-CoV-2 by RT-PCR on nasopharyngeal and oropharyngeal swabs from 7 December 2021 to 12 January 2022. Samples from individuals with a Ct value ≤ 33 were selected for the variants-specific mutation screening. The screening was performed using two kits, “SNPsig® SARS-COV-2 (Escape PLEX)" and "SNPsig® VariPLEX™ (COVID-19) Real-Time PCR Assay". Results: SARS-CoV-2 prevalence was 18.9% (332/1758). A total of 113 samples (34.04%) had a Ct value less than (≤ 33), with only 20.35% (23/113) belonging to symptomatic patients. The mean age was 39.01±13 years. The Beta variant (B.1.351) was the most detected one comprising 78.8% (89/113) of variants. Gamma and Delta variants were detected at a low proportion of 0.9% (1/113). No mutation or variant was detected in seven (6.2%) samples. Conclusion: Specific mutation screening detected Beta (B.1.351), Gamma (P.1), and Delta (B.1.617.2) variants of SARS-CoV-2 circulating in Burkina Faso. The absence of mutations in some samples might suggest variants other than those detected.
 
Keywords: SARS-CoV-2, RT-PCR, Ct, Variant, Mutation, Burkina Faso
Full-Text [PDF 803 kb]   (569 Downloads)    
Type of Study: Original article | Subject: Diagnostic/screening methods and protocols
Received: 2022/07/1 | Accepted: 2022/09/19 | Published: 2022/10/12
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.