Volume 10, Issue 3 (9-2022)                   JoMMID 2022, 10(3): 114-121 | Back to browse issues page

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Malaki A, Ferdosi-Shahandashti E, Maali A, Sabbagh P, Khademian A. Molecular Characterizations and Antimicrobial Susceptibility of Extended-Spectrum ß-lactamase (ESBL) Producing Proteus spp. Clinical Isolates in Babol, Northern Iran. JoMMID 2022; 10 (3) :114-121
URL: http://jommid.pasteur.ac.ir/article-1-437-en.html
Molecular Characterizations and Antimicrobial Susceptibility of Extended-Spectrum ß-lactamase (ESBL) Producing Proteus spp. Clinical Isolates in Babol, Northern Iran
Atena Malaki , Elaheh Ferdosi-Shahandashti , Amirhosein Maali , Parisa Sabbagh , Aynaz Khademian
Department of Medical Biotechnology, School of Medicine, Babol University of Medical Sciences, Babol, Iran; Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
Abstract:   (1011 Views)
Introduction: Proteus spp. are opportunistic members of Enterobacteriaceae, accounting for 10% of urinary tract infections and other primary clinical infections. They produce extended-spectrum beta-lactamases (ESBL) that can confer resistance to beta-lactam antibiotics. This study aimed to investigate the prevalence, antimicrobial susceptibility, molecular characteristics, and genetic relationship of ESBL-producing Proteus spp. clinical isolates in Babol, Northern Iran. Methods: In this cross-sectional study, out of 112 clinical samples, 30 Proteus spp. isolates were identified via specific biochemical assays. According to the Clinical and Laboratory Standards Institute (CLSI) guidelines, antibiotic susceptibility was evaluated using disc diffusion and agar dilution methods, and polymerase chain reaction (PCR) was used to detect blaTEM and blaSHV genes. Results: The resistance rate to tetracycline and sulfamethoxazole was highest by disk diffusion and agar dilution. Multiple drug-resistant (MDR) isolates were 86% and 60% in disk diffusion and agar dilution assays. Seven (23.3%) isolates had the blaTEM genes and 18 (60%) blaSHV. Conclusion:  ESBL-producing Proteus spp. was highly prevalent, and the blaSHV was the most common resistance contributing gene. These findings and relatively high resistance to ampicillin demand more care in prescribing antibiotics. Also, the high prevalence of MDR isolates in patients infected with ESBL-producing Proteus spp. requires continuous surveillance.
Keywords: Proteus spp, Beta-lactamase, Multi-drug resistance, Antibiotic resistance
Full-Text [PDF 988 kb]   (321 Downloads)    
Type of Study: Original article | Subject: Anti-microbial agents, resistance and treatment protocols
Received: 2021/12/19 | Accepted: 2022/09/19 | Published: 2022/10/12
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.