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Heydari H, Majd A, Hamidi-Fard M, Bahramali G, Aghasadeghi M R. Comparison of PCR with Serology for Detecting Acute Hepatitis A Virus Infection. JoMMID 2021; 9 (2) :82-87
URL: http://jommid.pasteur.ac.ir/article-1-336-en.html
Comparison of PCR with Serology for Detecting Acute Hepatitis A Virus Infection
Hossein Heydari , Ahmad Majd , Mojtaba Hamidi-Fard * , Golnaz Bahramali , Mohammad Reza Aghasadeghi
Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran & Viral Vaccine Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (2056 Views)
Introduction: Early detection of acute Hepatitis A virus infection (HAV) allows adopting proper treatment measures, rapid recovery, and avoiding side effects. This study compares PCR assay with serology for diagnosing acute HAV infection. Methods: Twenty samples from patients presenting clinical symptoms of acute hepatitis were tested for anti-HAV IgM antibodies. Genomic RNA was extracted from IgM-positive samples, cDNA was synthesized and examined for genomic HAV using a specific HAV real-time detection kit and a nested PCR. Results: Among 20 sera, 14 were positive for anti-HAV IgM antibodies. The specific real-time PCR and nested PCR showed agreement, and both detected HAV genetic material in 3 out of 14 samples. Conclusion: High levels of anti-HAV IgM antibodies do not necessarily indicate acute HAV infection in people presenting clinical symptoms of the disease.  Measuring IgM antibody levels alongside molecular detection of virus genome by DNA-based methods assay can lead to an accurate, timely, and reliable diagnosis of active HAV infection.
Keywords: Molecular diagnostic testing, Serological tests, Hepatitis A viruses
Full-Text [PDF 928 kb]   (1032 Downloads)    
Type of Study: Original article | Subject: Diagnostic/screening methods and protocols
Received: 2021/02/6 | Accepted: 2021/06/20 | Published: 2021/08/29
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.