Volume 9, Issue 2 (6-2021)                   JoMMID 2021, 9(2): 82-87 | Back to browse issues page

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Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran & Viral Vaccine Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (394 Views)
Introduction: Early detection of acute Hepatitis A virus infection (HAV) allows adopting proper treatment measures, rapid recovery, and avoiding side effects. This study compares PCR assay with serology for diagnosing acute HAV infection. Methods: Twenty samples from patients presenting clinical symptoms of acute hepatitis were tested for anti-HAV IgM antibodies. Genomic RNA was extracted from IgM-positive samples, cDNA was synthesized and examined for genomic HAV using a specific HAV real-time detection kit and a nested PCR. Results: Among 20 sera, 14 were positive for anti-HAV IgM antibodies. The specific real-time PCR and nested PCR showed agreement, and both detected HAV genetic material in 3 out of 14 samples. Conclusion: High levels of anti-HAV IgM antibodies do not necessarily indicate acute HAV infection in people presenting clinical symptoms of the disease.  Measuring IgM antibody levels alongside molecular detection of virus genome by DNA-based methods assay can lead to an accurate, timely, and reliable diagnosis of active HAV infection.
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Type of Study: Original article | Subject: Diagnostic/screening methods and protocols
Received: 2021/02/6 | Accepted: 2021/06/20 | Published: 2021/08/29

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