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Safaei S, Rezvan H, Fateh R, Khalifeh Gholi M. Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit. JoMMID. 2020; 8 (2) :60-64
URL: http://jommid.pasteur.ac.ir/article-1-246-en.html
Department of Pathobiology, School of Paraveterinary Sciences, BU-Ali Sina University, Hamadan, Iran.
Abstract:   (285 Views)
Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone. Methods: The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70  (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit’s serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.
Full-Text [PDF 489 kb]   (100 Downloads)    
Type of Study: Original article | Subject: Diagnostic/screening methods and protocols
Received: 2020/04/25 | Accepted: 2020/04/13 | Published: 2020/08/16

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