Volume 8, Issue 2 (4-2020)                   JoMMID 2020, 8(2): 60-64 | Back to browse issues page

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Department of Pathobiology, School of Paraveterinary Sciences, BU-Ali Sina University, Hamadan, Iran.
Abstract:   (962 Views)
Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone. Methods: The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70  (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit’s serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.
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Type of Study: Original article | Subject: Diagnostic/screening methods and protocols
Received: 2020/04/25 | Accepted: 2020/04/13 | Published: 2020/08/16

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