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Ghafari S M, Ebadatgar V, Mohammadi S, Ebrahimi S, Bordbar A, Parvizi P. Morphologic, Morphometric and Molecular Comparison of Two Sister Species of Rodents as Potential Reservoir Hosts of Zoonotic Cutaneous Leishmaniasis in the Southwest of Iran. JoMMID 2019; 7 (3) :79-84
URL: http://jommid.pasteur.ac.ir/article-1-209-en.html
Morphologic, Morphometric and Molecular Comparison of Two Sister Species of Rodents as Potential Reservoir Hosts of Zoonotic Cutaneous Leishmaniasis in the Southwest of Iran
Seyedeh Maryam Ghafari , Vahoora Ebadatgar , Somayeh Mohammadi , Sahar Ebrahimi , Ali Bordbar , Parviz Parvizi *
Molecular Systematics Laboratory, Department of Parasitology , Pasteur Institute of Iran, Tehran, Iran
Abstract:   (4779 Views)
Introduction: Rodents are reservoir hosts of various infectious diseases. Many species and subspecies of genus Rattus play a significant role as potential reservoir hosts of different emerging and re-emerging diseases, including leishmaniasis. Methods: Rodents were captured using live wooden traps from different localities of Khuzestan Province, southwest of Iran. To precise identification of two sister species of rats, including Rattus rattus and Rattus norvegicus, morphological, molecular, and biosystematics characters were examined using amplification of mitochondrial Cytochrome b (Cytb) gene fragment. Results: Out of 119 captured rodents, 44 were R. rattus, 12 were R. norvegicus, and 63 belonged to other species (Tatera indica, Nesokia indica, Mus musculus). Partial Cyt b gene (≤624 bp) was amplified to characterize R. rattus and R. norvegicus, accurately. Three haplotypes of R. rattus (six samples) and a unique haplotype of R. norvegicus (three samples) were identified with some nucleotide variations. Conclusion: Mitochondrial results confirmed morphological disparity between the two Rattus species in Khuzestan Province. Therefore, we recommend applying an integrative approach to identify host reservoirs for infectious diseases, especially those suspected as reservoirs of cutaneous Leishmaniasis.
Keywords: Morphology, Cytochrome b, Rattus rattus, Rattus norvegicus.
Full-Text [PDF 598 kb]   (722 Downloads)    
Type of Study: Original article | Subject: Infectious diseases and public health
Received: 2019/07/24 | Accepted: 2019/11/10 | Published: 2020/03/9
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.