:: Volume 5, Issue 3 And 4 (7-2017) ::
JoMMID 2017, 5(3 And 4): 40-42 Back to browse issues page
Detection of Brucella spp. in the Sheep and Goats Milks from Southeastern Iran Using Culture and PCR
Zahra Shirazi , Mohammad Khalili , Balal Sadeghi , Hamid Sharifi , ShahrnazBanuo Ashrafganjooyi
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Abstract:   (950 Views)
Introduction: Brucellosis is a zoonotic disease caused by the members of the genus Brucella. This bacterium is transmitted to humans through exposure to infected animals or via consumption of contaminated dairy products. Cultivation of the bacteria or amplification of its DNA using polymerase chain reaction (PCR) are conventional diagnostic approaches for definitive identification of Brucella in raw milk. Method: We collected 530 milk samples from 485 healthy animals, and 45 animals with a history of abortion from Kerman province, southeast Iran. The specimens were first cultured in Eugon broth and then were subcultured on Brucella agar. Gram smears from colonies for characterization of the bacteria were prepared. Also, DNA extraction and PCR amplification of IS711 fragment were performed to detect Brucella DNA in the milk samples. Results: The culture method detected Brucella Spp. in 10 milk samples including two samples from apparently healthy animals (1 sheep sample, and 1 goat sample) as well as eight samples from animals with abortion history (6 sheep samples, and 2 goat samples). PCR identified Brucella DNA in 43 samples including those from healthy sheep (n=4) and goats (n=9), as well as animals milk with abortion history (7 sheep, and 23 goats). The proportion of positive samples detected by PCR method was significantly higher than culture method (P=0.014). Conclusion: The PCR assay turned to be a convenient method for detection of Brucella contamination of raw milk and can be used as a reliable tool for surveillance and screening of contaminated milk.
Keywords: Brucella, PCR, Culture, Goat, Sheep.
Full-Text [PDF 335 kb]   (194 Downloads)    
Type of Study: Original article | Subject: Microbial pathogenesis
Received: 2017/12/31 | Accepted: 2018/02/3 | Published: 2018/03/14
References
1. ------------- 1. Gwida M, Al Dahouk S, Melzer F, Rösler U, Neubauer H, Tomaso H. Brucellosis - regionally emerging zoonotic disease? Croat Med J. 2010; 51 (4): 289-95. [DOI:10.3325/cmj.2010.51.289] [PMID] [PMCID]
2. 2. Al Dahouk S, Nöckler K. Implications of laboratory diagnosis on brucellosis therapy. Expert Rev Anti Infect Ther. 2011; 9 (7): 833-45. [DOI:10.1586/eri.11.55] [PMID]
3. 3. ARASOĞLU T, GÜLLÜCE M, ÖZKAN H, ADIGÜZEL A, ŞAHİN F. PCR detection of Brucella abortus in cow milk samples collected from Erzurum, Turkey. Turkish Journal of Medical Sciences. 2013; 43 (4): 501-8. [DOI:10.3906/sag-1205-121]
4. 4. Addis M. Public Health and Economic Importance of Brucellosis: A Review. Public Health. 2015; 5 (7): 68-84.
5. 5. Sambrook J, Russell, DW. The condensed protocols from molecular cloning: a laboratory manual. 1st ed. New York, Cold Spring Harbor Laboratory Press. 2006; 39-45.
6. 6. Ning P, Guo K, Xu L, Xu R, Zhang C, Cheng Y, et al. Short communication: Evaluation of Brucella infection of cows by PCR detection of Brucella DNA in raw milk. J Dairy Sci. 2012; 95 (9): 4863-7. [DOI:10.3168/jds.2012-5600] [PMID]
7. 7. Hamdy M, Amin A. Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Vet J. 2002; 163 (3): 299-305. [DOI:10.1053/tvjl.2001.0681] [PMID]
8. 8. Ilhan Z, Solmaz H, Aksakal A, Gulhan T, Ekin I, Boynukara B. Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay. Arch Med Vet. 2008; 40 (2): 141-6. [DOI:10.4067/S0301-732X2008000200005]
9. 9. Baron S. Epidemiology--Medical Microbiology: University of Texas Medical Branch at Galveston; 1996.



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