:: Volume 5, Issue 1 And 2 (1-2017) ::
JoMMID 2017, 5(1 And 2): 31-34 Back to browse issues page
Trichosomoides crassicauda Infection in Laboratory Rats with Histopathological Description in the Bladder Tissue
Faezeh Najafi , Maede Taghavi Ghadikolai , Saied Reza Naddaf , Hamid Hasanpour , Iraj Mobedi , Gholamreza Mowlavi
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (1386 Views)
Introduction: Some postgraduate students reported the presence of a nematode infection, presumably, Trichosomoides crassicauda in the urinary bladder of rats (Rattus norvegicus) from the animal house of Tehran University of Medical Sciences (TUMS). We prompted to explore the prevalence of this infection among the rats of animal houses belonging to medical and veterinary research centers in Tehran. We also described the histopathological changes in the bladder tissue of the infected rats. Methods: We collected 214 adult rats (R. norvegicus) from four animal houses including TUMS in Tehran. The urine of 112 animals was collected during 24 h and inspected microscopically for helminths eggs. Then, the rats were euthanized, sacrificed and dissected; their bladders were removed and examined under a stereomicroscope for the presence of worms and histopathological changes. Results: Out of 112 urine samples checked, 16 contained T. crassicauda eggs. Out of 214 rats, 114 showed infection urinary bladder with the nematode T. crassicauda. The histopathological changes in the urinary bladder included hyperemia, edema, hyperplasia, and metaplasia. In the transitional epithelium, the features of gravid female nematodes, with an egg-filled uterus were visible. Conclusion: Trichosomoides crassicauda is a prevalent infection in laboratory rats of research centers in Tehran and its identification cannot be made merely based on urine examination of the rats.
Keywords: Trichosomoides crassicauda, Laboratory Rats, Histopathological Changes
Full-Text [PDF 441 kb]   (338 Downloads)    
Type of Study: Original article | Subject: Animal Models
Received: 2017/10/9 | Accepted: 2017/12/9 | Published: 2017/12/27
References
1. 1. Perec-Matysiak A, Okulewicz A, Hildebrand J, Zaleśny G. Helminth parasites of laboratory mice and rats. Wiad Parazytol. 2005; 52 (2): 99-102.
2. Gill TJ, Smith GJ, Wissler RW, Kunz HW. The rat as an experimental animal. Science. 1989; 245 (4915): 269-76. [DOI:10.1126/science.2665079] [PMID]
3. Najafi F, Rezaie S, Kia E, Mobedi I, Mahmodi M, Salimi M, Hasanpour H, Makki M, Mowlavi G. Intestinal helminths in laboratory mice and rats in four research centers, Tehran, Iran. J Med Microbiol Infec Dis. 2014; 2 (4): 130-2.
4. Bellingham O. Catalogue of entozoa indigenous to Ireland, with observations. Annals of the Magazine of Natural History. 1840; 4: 343-51.
5. Ozkorkmaz EG. Microscopic investigations in a diabetic rat urinary bladder infected with Trichosomoides crassicauda. J Electron Microsc (Tokyo). 2011; 60 (4): 261-5. [DOI:10.1093/jmicro/dfr012] [PMID]
6. Kia E, Homayouni M, Farahnak A, Mohebali M, Shojai S. Study of endoparasites of rodents and their zoonotic importance in Ahvaz, south west Iran. Iranian J Publ Health. 2001; 30 (1-2): 49-52.
7. Antonakopoulos G, Turton J, Whitfield P, Newman J. Host-parasite interface of the urinary bladder-inhabiting nematode Trichosomoides crassicauda: changes induced in the urothelium of infected rats. Int J Parasitol. 1991; 21 (2): 187-93. [DOI:10.1016/0020-7519(91)90009-V]
8. Yokogawa S. On the migratory course of Trichosomoides crassicauda (Bellingham) in the body of the final host. J Parasitol. 1920; 7 (2): 80-4. [DOI:10.2307/3270959]
9. Zubaidy AJ, Majeed SK. Pathology of the nematode Trichosomoides crassicauda in the urinary bladder of laboratory rats. Lab Anim. 1981; 15 (4): 381-4. [DOI:10.1258/002367781780952915] [PMID]
10. Chapman W. Infection with Trichosomoides crassicauda as a factor in the induction of bladder tumors in rats fed 2-acetylaminofluorene. Invest Urol. 1969; 7 (2): 154-9. [PMID]
11. Flynn RJ. Parasites of laboratory animals. 1st ed. Iowa state University Press: Ames; 1973; 345-6.
12. Hernández-Bello R, Ramirez-Nieto R, Mu-iz-Hernández S, Nava-Castro K, Pavón L, Sánchez-Acosta AG, Morales-Montor J. Sex steroids effects on the molting process of the helminth human parasite Trichinella spiralis. J Biomed Biotechnol. 2011; 1-10. [DOI:10.1155/2011/625380] [PMID] [PMCID]
13. Cornish J, Vanderwee M, Findon G, Miller T. Reliable diagnosis of Trichosomoides crassicauda in the urinary bladder of the rat. Lab Anim. 1988; 22 (2): 162-5. [DOI:10.1258/002367788780864385] [PMID]
14. Weisbroth S, Scher S. Trichosomoides crassicauda infection of a commercial rat breeding colony. I. Observations on the life cycle and propagation. Lab Anim Sci. 1971; 21 (1): 54-61. [PMID]
15. Bahrami S, Rezaei A, Alborzi A. Trichosomoides crassicauda infection in wistar rats. Arch Razi Inst. 2014; 69 (1): 63-7.
16. Tabaran F, Catoi C, Gal A, Bolfă P, Taulescu M, Nagy A, Cuc C, Borza G, Moussa R. Histopathological changes of the urinary bladder in the natural occurred infection with Trichosomoides crassicauda in Albino rats. Bulletin UASVM. 2011; 68 (1): 401.
17. Serakides R, Ribeiro A, Silva C, Santos R, Nunes V, Nascimento E. Proliferative and inflammatory changes in the urinary bladder of female rats naturally infected with Trichosomoides crassicauda: report of 48 cases. Arq Bras Med Vet Zootec. 2001; 53 (2): 1-5. [DOI:10.1590/S0102-09352001000200012]
18. Thomas LJ. Studies on the life history of Trichosomoides crassicauda (Bellingham). J Parasitol. 1924; 10 (3): 105-36. [DOI:10.2307/3270872]
19. Chapman WH. The incidence of a nematode, Trichosomoides crassicauda, in the bladder of laboratory rats. treatment with nitrofurantoin and preliminary report of their influence on urinary calculi and experimental bladder tumors. Invest Urol. 1964; 2: 52-7. [PMID]
20. Martin B. Cell replacement and differentiation in transitional epithelium: a histological and autoradiographic study of the guinea-pig bladder and ureter. J Anat. 1972; 112 (3): 433-55. [PMID] [PMCID]



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