Volume 5, Issue 1 And 2 (1-2017)                   JoMMID 2017, 5(1 And 2): 17-20 | Back to browse issues page

XML Print

Department of Biology,Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract:   (4724 Views)
Introduction: Urinary tract infection (UTI) is one of the most common infectious diseases, and Escherichia coli is known as the most dominant causative agent of this infection in 90-80% of patients. There is not much information about the phylogenetic groups, types, and virulence factors of E. coli, causing UTIs from Iran. The objective of this study was to evaluate the antimicrobial susceptibility of E. coli strains isolated from patients with UTI and to determine the phylogenetic classification of the strains. Methods: In this cross-sectional study, 100 E. coli isolates were collected from patients with UTI from five laboratories in Rasht city. The isolates were confirmed by using morphological and biochemical common tests. The frequency of virulence genes and patterns of phylogenetic groups were performed using Multiplex PCR. Additionally, antimicrobial susceptibility of all isolates was evaluated by disk diffusion method. Results: Distribution of phylogenetic B2, D, A and B1 groups in the isolates were 64%, 24%, 12%, and 0%, respectively. The highest antibiotic resistance was reported to cefotaxime (84%), piperacillin, and cefixime (80%), and the lowest resistance was demonstrated to imipenem (8%), chloramphenicol and gentamicin (12%). Conclusion: Our findings showed that the B2 was the most prevalent phylogenetic group and the most resistant strain to generally used antibiotics among patients with UTI.
Full-Text [PDF 615 kb]   (1060 Downloads)    
Type of Study: Original article | Subject: Epidemiologic studies including microbial genotyping, phenotyping and serotyping
Received: 2017/07/21 | Accepted: 2017/12/30 | Published: 2018/01/8

1. 1. Petkovsek Z, Elersic K, Gubina M, Zgur-Bertok D, Starcic Erjavcc M. Virulence potential of Escherichia coli isolates from skin and soft tissue infections. J Clin Microbiol. 2009; 47 (6): 1811-7. [DOI:10.1128/JCM.01421-08] [PMID] [PMCID]
2. Rijavec M, Muller-Premru M, Zakotnik B, Zgur- Bertok D. Virulence factors and biofilm production among Escherichia coli strains causing bacteremia of urinary tract origin. J Med Microbiol. 2008; 57 (11): 1329-34. [DOI:10.1099/jmm.0.2008/002543-0] [PMID]
3. Saeed MA, Haque A, Ali A, Mohsin M, Bashir S, Tariq A, Afzal A, Iftikhar T, Sarwar Y. Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections. J Infect Dev Ctries. 2009; 3 (9): 667-70. [PMID]
4. Soleimani N, Aganj M, Ali L, Shokoohizadeh L, Sakinc T. Frequency distribution of genes encoding aminoglycoside modifying enzymes in uropathogenic E. coli isolated from Iranian hospital. BMC Res Notes. 2014; 7: 842. [DOI:10.1186/1756-0500-7-842] [PMID] [PMCID]
5. Bonacorsi SP, Clermont O, Tinsley C, Le Gall I, Beaudoin JC, Elion J, Nassif X, Bingen E. Identification of regions of the Escherichia coli chromosome specific for neonatal meningitis associated strains. Infect Immun. 2000; 68 (4): 2096-101. [DOI:10.1128/IAI.68.4.2096-2101.2000] [PMID] [PMCID]
6. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol. 2000; 66 (10): 4555-8. [DOI:10.1128/AEM.66.10.4555-4558.2000] [PMID] [PMCID]
7. Blattner FR, Plunkett GI, Bloch CA, Perna NT, Burland V, Riley M, et al. The complete genome sequence of Escherichia coli K-12. Science. 1997; 277 (5331): 1453-62. [DOI:10.1126/science.277.5331.1453] [PMID]
8. Torres AG, Payne S. Haem iron-transport system in enterohaemorrhagic Escherichia coli O157:H7. Mol Microbiol. 1997; 23(4): 825-33. [DOI:10.1046/j.1365-2958.1997.2641628.x]
9. Johnson JR, Kuskowski MA, Owens K, Gajewski A, Winokur PL. Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans. J Infect Dis. 2007; 188 (5): 759-68. [DOI:10.1086/377455] [PMID]
10. Grude N, Potaturkina-Nesterova N I., Jenkins A, Strand L, Nowrouzian F L, Nyhus J, Kristiansen BE. A comparison of phylogenetic group, virulence factors and antibiotic resistance in Russian and Norwegian isolates of Escherichia coli from urinary tract infection. Clin Microbial Infect. 2007; 13 (2): 208-11. [DOI:10.1111/j.1469-0691.2006.01584.x] [PMID]
11. Sawma-Aouad G, Hashwa F, Tokajian S. Antimicrobial resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli in Labanon. J Chemother. 2009; 21 (2): 153-8. [DOI:10.1179/joc.2009.21.2.153] [PMID]
12. Bashir S, Haque A, Sarwar Y, Ali A, Anwar MI. Virulence profile of different phylogenetic groups of locally isolated community acquired uropathogenic E. coli from Faisalabad region of Pakistan. Ann Clin Microbiol Antimicrob. 2012; 11: 23-9. [DOI:10.1186/1476-0711-11-23] [PMID] [PMCID]
13. Abdi HA, Ghalehnoo MR. Virulence genes, genetic diversity, antimicrobial susceptibility and phylogenetic background of Escherichia coli isolates. Int J Enter Pathog. 2015; 3: 25692. [DOI:10.17795/ijep.25692]
14. Iranpour D, Hassanpour M, Ansari H, Tajbakhsh S, Khamisipour GR, Najafi A. Phylogenetic Groups of Escherichia coli Strains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method. BioMed Res Int. 2015, Article ID 846219.
15. Zhao L, Chen X, Zhu X, Yang W, Dong L, Xu X, Gao S, Liu X. Prevalence of virulence factors and antimicrobial resistance of uropathogenic Escherichia coli in Jiangsu province (China). Urology. 2009; 74 (3): 702-7. [DOI:10.1016/j.urology.2009.01.042] [PMID]
16. Moreno E, Prats G, Sabate M, Perez T, Johnson J R, Andreu A. Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli. J Antimicrob Chemother. 2006; 57: 204-11. [DOI:10.1093/jac/dki468] [PMID]
17. Johnson JR, Kuskowski MA, Gajewski A, Soto S, Horcajada JP, Jimenez de Anta MT, et al. Extended virulence genotypes and phylogenetic background of Escherichia coli isolates from patients with cystitis, pyelonephritis, or prostatitis. J Infect Dis. 2005; 191 (1): 46-50. [DOI:10.1086/426450] [PMID]
18. Asadi S, Solhjoo K, Kargar M, Rezaeian A. Phylogenetic groups of Escherichia coli stains Isolated from urinary tract infection in jahrom city, southern Iran. J Microbiol World. 2011; 13 (4): 245-50.
19. Derakhshandeh A, Firouzi R, Moatamedifar M, Motamedi A, Bahadori M, Naziri Z. Phylogenetic analysis of Escherichia coli strains isolated from human samples. Mol Biol Res Com. 2013; 2 (4): 143-9.
20. Hussain A, Ranjan A, Nandanwar N, Babbar A, Jadhav S, Ahmed N. Genotypic and Phenotypic Profiles of Escherichia coli Isolates Belonging to Clinical Sequence Type 131 (ST131), Clinical Non-ST131, and Fecal Non-ST131 Lineages from India. Antimicrob Agents Chemother. 2014; 15 (12): 7240-9. [DOI:10.1128/AAC.03320-14] [PMID] [PMCID]
21. Karami N, Wold AE, Adlerberth I. Antibiotic resistance is linked to carriage of papC and iutA virulence genes and phylogenetic group D background in commensal and uropathogenic Escherichia coli from infants and young children. Eur J Clin Microbiol Infect Dis. 2017; 36 (4): 721-9. [DOI:10.1007/s10096-016-2854-y] [PMID] [PMCID]