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JoMMID 2017, 5(1 And 2): 1-11 Back to browse issues page
An Overview on Leishmania Diagnosis
Hossein Rezvan , Ali reza Nourian , Sahar Hamoon Navard
Department of Pathobiology School of Veterinary Science Bu-Ali Sina University Hamedan, Iran
Abstract:   (1252 Views)
Leishmaniasis is now accounted as a health problem and categorized as a class I disease (emerging and uncontrolled) by World Health Organization (WHO), causing highly significant morbidity and mortality with different clinical presentations. The incidence of human leishmaniasis is increasing and its geographic distribution in humans and animals is shown to be wider than estimated before. Indeed, more than 350 million people are at risk of Leishmania infection, and about 1.6 million new cases occur causing more than 50 thousand death annually. Control of leishmaniasis is highly dependent to the early diagnosis and treatment of the disease. In recent years, there have been advances in diagnosis of Leishmania infection. However, the main challenge in Leishmania diagnosis is the lack of a gold standard test in order to establish an effective strategic program to control and eradicate the disease. This review provides the latest information regarding the diagnosis of the disease, which is based on a combination of clinical features (supported by epidemiologic data) and laboratory tests including direct parasitological (microscopy, histopathology, and parasite culture), serological and molecular tests.
Keywords: Leishmaniasis, Leishmania, diagnosis
Full-Text [PDF 573 kb]   (232 Downloads)    
Type of Study: Review article | Subject: Infectious diseases and public health
Received: 2017/04/15 | Accepted: 2018/02/7 | Published: 2018/02/7
References
1. Hotez PJ, Molyneux DH, Fenwick A, Ottesen E, Ehrlich Sachs S, Sachs JD. Incorporating a rapid-impact package for neglected tropical diseases with programs for HIV/AIDS, tuberculosis, and malaria. PLoS Med. 2006; 3 (5): e102. [DOI:10.1371/journal.pmed.0030102] [PMID] [PMCID]
2. Karami M, Doudi M, Setorki M. Assessing epidemiology of cutaneous leishmaniasis in Isfahan, Iran. J Vector Borne Dis. 2013; 50 (1): 30-7. [PMID]
3. Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PloS one. 2012; 7 (5): e35671. [DOI:10.1371/journal.pone.0035671] [PMID] [PMCID]
4. Ponte-Sucre A, Diaz E, Padrón-Nieves M. Drug resistance in leishmania parasites. New York, USA: Springer. 2013; 65-83. [DOI:10.1007/978-3-7091-1125-3]
5. Rezvan H, Rees RC, Ali SA. Development of A peptide-based sub-unit vaccine by selecting peptides from the essential surface glycoproteins of Leishmania parasites, Gp63, Using transgenic mouse model. In proceedings: 12th Iranian reserchers conference in Europe. Manchester, UK: 2003; 52.
6. WHO. Leishmaniasis. [updated 2017 April; cited 2017 December 25]; Available from: http://www.who.int/-mediacentre/factsheets-/fs375/en/.
7. Nagayoshi Y, Miyazaki T, Minematsu A, Hosogaya N, Morinaga Y, Nakamura S, et al. P245 Unexpected effects of the monoamine oxidase A inhibitor clorgyline on antifungal susceptibility of Candida glabrata. Int J Antimicrob Agents. 2013; 42: S119-20. [DOI:10.1016/S0924-8579(13)70486-2]
8. Jimenez M, Gonzalez E, Iriso A, Marco E, Alegret A, Fuster F, et al. Detection of Leishmania infantum and identification of blood meals in Phlebotomus perniciosus from a focus of human leishmaniasis in Madrid, Spain. Parasitol Res. 2013; 112 (7): 2453-9. [DOI:10.1007/s00436-013-3406-3] [PMID]
9. Temeyer KB, Brake DK, Tuckow AP, Li AY, Pérez deLeón AA. Acetylcholinesterase of the sand fly, Phlebotomus papatasi (Scopoli): cDNA sequence, baculovirus expression, and biochemical properties. Parasit Vectors. 2013; 6 (1): 31. [DOI:10.1186/1756-3305-6-31] [PMID] [PMCID]
10. Campino L, Cortes S, Dionisio L, Neto L, Afonso MO, Maia C. The first detection of Leishmania major in naturally infected Sergentomyia minuta in Portugal. Mem Inst Oswaldo Cruz. 2013; 108 (4): 516-8. [DOI:10.1590/S0074-02762013000400020] [PMID] [PMCID]
11. McCarthy CB, Santini MS, Pimenta PF, Diambra LA. First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis. PloS one. 2013; 8 (3): e58645. [DOI:10.1371/journal.pone.0058645] [PMID] [PMCID]
12. Afsahi A, Aeini Z, Rezvan H, Aazami S. Studies on using cattle and sheep hydatid cyst fluid instead of the fetal calf serum in Leishmania culture. ZJRMS. 2013; 15 (12): 9-12.
13. Ali SA, Rezvan H, Khodadadi A, McArdle SEB, Rees RC. A mouse model to evaluate the role of CTLs in Leishmania DNA vaccines. In proceedings: Spring trypanosomiasis/-leishmaniasis and malaria meetings. Newcastle, UK: 2008; 129.
14. Engwerda CR, Ato M, Kaye PM. Macrophages, pathology and parasite persistence in experimental visceral leishmaniasis. Trends Parasitol. 2004; 20 (11): 524-30. [DOI:10.1016/j.pt.2004.08.009] [PMID]
15. Asfaram S, Fakhar M, Mohebali M, Mardani A, Banimostafavi ES, Hezarjaribi HZ, et al. Asymptomatic human blood donors carriers of Leishmania infantum: potential reservoirs for visceral leishmaniasis in northwestern Iran. Transfus Apher Sci. 2017; 56 (3): 474-9. [DOI:10.1016/j.transci.2017.06.001] [PMID]
16. Cardo LJ. Leishmania: risk to the blood supply. Transfusion. 2006; 46 (9): 1641-5. [DOI:10.1111/j.1537-2995.2006.00941.x] [PMID]
17. Singh S. New developments in diagnosis of leishmaniasis. Indian J Med Res. 2006; 123 (3): 311-30. [PMID]
18. Karamian M, Kuhls K, Hemmati M, Ghatee MA. Phylogenetic structure of Leishmania tropica in the new endemic focus Birjand in East Iran in comparison to other Iranian endemic regions. Acta tropica. 2016; 158: 68-76. [DOI:10.1016/j.actatropica.2016.02.010] [PMID]
19. Zijlstra EE, Musa AM, Khalil EA, el-Hassan IM, el-Hassan AM. Post-kala-azar dermal leishmaniasis. Lancet Infect Dis. 2003; 3 (2): 87-98. [DOI:10.1016/S1473-3099(03)00517-6]
20. Johnson P, Jones CT, Kendall JZ, Ritchie JW, Thorburn GD. ACTH and the induction of parturition in sheep. J Physiol. 1975; 252 (2): 64-6.
21. Zijlstra EE. PKDL and other dermal lesions in HIV co-infected patients with Leishmaniasis: review of clinical presentation in relation to immune responses. PLoS Negl Trop Dis. 2014; 8 (11): e3258. [DOI:10.1371/journal.pntd.0003258] [PMID] [PMCID]
22. van Griensven J, Carrillo E, Lopez-Velez R, Lynen L, Moreno J. Leishmaniasis in immunosuppressed individuals. Clin Microbiol Infect. 2014; 20 (4): 286-99. [DOI:10.1111/1469-0691.12556] [PMID]
23. Robbins K, Khachemoune A. Cutaneous myiasis: a review of the common types of myiasis. Int J Dermatol. 2010; 49 (10): 1092-8. [DOI:10.1111/j.1365-4632.2010.04577.x] [PMID]
24. Maurya R, Singh RK, Kumar B, Salotra P, Rai M, Sundar S. Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure. J Clin Microbiol. 2005; 43 (7): 3038-41. [DOI:10.1128/JCM.43.7.3038-3041.2005] [PMID] [PMCID]
25. Herwaldt BL. Leishmaniasis. Lancet. 1999; 354 (9185): 1191-9. [DOI:10.1016/S0140-6736(98)10178-2]
26. Guerin PJ, Olliaro P, Sundar S, Boelaert M, Croft SL, Desjeux P, et al. Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda. Lancet Infect Dis. 2002; 2 (8): 494-501. [DOI:10.1016/S1473-3099(02)00347-X]
27. Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, et al. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol. 2007; 5 (11): 873-82. [DOI:10.1038/nrmicro1748] [PMID]
28. van den Bogaart E, Berkhout MM, Nour AB, Mens PF, Talha AB, Adams ER, et al. Concomitant malaria among visceral leishmaniasis in-patients from Gedarif and Sennar States, Sudan: a retrospective case-control study. BMC public health. 2013; 13: 332. [DOI:10.1186/1471-2458-13-332] [PMID] [PMCID]
29. El Hajj L, Thellier M, Carriere J, Bricaire F, Danis M, Caumes E. Localized cutaneous leishmaniasis imported into Paris: a review of 39 cases. Int J Dermatol. 2004; 43 (2): 120-5. [DOI:10.1111/j.1365-4632.2004.01991.x] [PMID]
30. Blum J, Desjeux P, Schwartz E, Beck B, Hatz C. Treatment of cutaneous leishmaniasis among travellers. J Antimicrob Chemother. 2004; 53 (2): 158-66. [DOI:10.1093/jac/dkh058] [PMID]
31. Weina PJ, Neafie RC, Wortmann G, Polhemus M, Aronson NE. Old world leishmaniasis: an emerging infection among deployed US military and civilian workers. Clin Infect Dis. 2004; 39 (11): 1674-80. [DOI:10.1086/425747] [PMID]
32. Santarém N, Cordeiro-da-Silva A. Diagnosis of visceral leishmaniasis. In: Mendez-Vilas A, editor. Communicating current research and educational topics and trends in applied microbiology. Badajoz: Formatex. 2007: 839-46.
33. Al-Hucheimi SN, Sultan BA, Al-Dhalimi MA. A comparative study of the diagnosis of Old World cutaneous leishmaniasis in Iraq by polymerase chain reaction and microbiologic and histopathologic methods. Int J Dermatol. 2009; 48 (4): 404-8. [DOI:10.1111/j.1365-4632.2009.03903.x] [PMID]
34. Rasti S, Ghorbanzadeh B, Kheirandish F, Mousavi SG, Pirozmand A, Hooshyar H, et al. Comparison of Molecular, Microscopic, and Culture Methods for Diagnosis of Cutaneous Leishmaniasis. J Clin Lab Anal. 2016; 30 (5): 610-5. [DOI:10.1002/jcla.21910] [PMID]
35. Blum J, Desjeux P, Schwartz E, Beck B, Hatz C. Treatment of cutaneous leishmaniasis among travellers. J Antimicrob Chemother. 2004; 53 (2): 158-66. [DOI:10.1093/jac/dkh058] [PMID]
36. Al-Jawabreh A, Schoenian G, Hamarsheh O, Presber W. Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears. Acta Trop. 2006; 99 (1): 55-61. [DOI:10.1016/j.actatropica.2006.07.001] [PMID]
37. Biddlestone LR, Hepburn NC, McLaren KM. A clinico-pathological study of cutaneous leishmaniasis in British troops from Belize. Trans R Soc Trop Med Hyg. 1994; 88 (6): 672-6. [DOI:10.1016/0035-9203(94)90223-2]
38. Singh A, Ramesh V. Histopathological features in leprosy, post-kala-azar dermal leishmaniasis, and cutaneous leishmaniasis. Indian J Dermatol Venereol Leprol. 2013; 79 (3): 360-6. [DOI:10.4103/0378-6323.110795] [PMID]
39. Farah F, Klaus S, Frankenburg S, Klion AD. Protozoan and helminth infections. Dermatology In General Medicine. 1993; 100: 2772-7.
40. Viana AG, Mayrink W, Fraga CA, Silva LM, Domingos PL, Bonan PR, et al. Histopathological and immunohistochemical aspects of American cutaneous leishmaniasis before and after different treatments. An Bras Dermatol. 2013; 88 (1): 32-40. [DOI:10.1590/S0365-05962013000100003] [PMID] [PMCID]
41. Mehregan DR, Mehregan DA, Mehregan AH. Histopathology of cutaneous leishmaniasis. Gulf J Dermatol Venereol. 1997; 4 (2): 1-9.
42. Handler MZ, Patel PA, Kapila R, Al-Qubati Y, Schwartz RA. Cutaneous and mucocutaneous leishmaniasis: Differential diagnosis, diagnosis, histopathology, and management. J Am Acad Dermatol. 2015; 73 (6): 911-26. https://doi.org/10.1016/j.jaad.2014.09.014 [DOI:10.1016/j.jaad.2014.08.051] [PMID]
43. Siddig M, Ghalib H, Shillington DC, Petersen EA. Visceral leishmaniasis in the Sudan: comparative parasitological methods of diagnosis. Trans R Soc Trop Med Hyg. 1988; 82 (1): 66-8. [DOI:10.1016/0035-9203(88)90265-9]
44. Markle WH, Makhoul K. Cutaneous leishmaniasis: recognition and treatment. Am Fam Physician. 2004; 69 (6): 1455-60. [PMID]
45. Andresen K, Gaafar A, El-Hassan AM, Ismail A, Dafalla M, Theander TG, et al. Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. Trans R Soc Trop Med Hyg. 1996; 90 (2): 133-5. [DOI:10.1016/S0035-9203(96)90112-1]
46. Disch J, Pedras MJ, Orsini M, Pirmez C, de Oliveira MC, Castro M, et al. Leishmania (Viannia) subgenus kDNA amplification for the diagnosis of mucosal leishmaniasis. Diagn Microbiol Infect Dis. 2005; 51 (3): 185-90. [DOI:10.1016/j.diagmicrobio.2004.10.005] [PMID]
47. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol. 2006; 44 (4): 1435-9. [DOI:10.1128/JCM.44.4.1435-1439.2006] [PMID] [PMCID]
48. Osman OF, Oskam L, Kroon NC, Schoone GJ, Khalil ET, El-Hassan AM, et al. Use of PCR for diagnosis of post-kala-azar dermal leishmaniasis. J Clin Microbiol. 1998; 36 (6): 1621-4. [PMID] [PMCID]
49. Allahverdiyev AM, Bagirova M, Uzun S, Alabaz D, Aksaray N, Kocabas E, et al. The value of a new microculture method for diagnosis of visceral leishmaniasis by using bone marrow and peripheral blood. Am J Trop Med Hyg. 2005; 73 (2): 276-80. [PMID]
50. Osman OF, Oskam L, Zijlstra EE, Kroon NC, Schoone GJ, Khalil ET, et al. Evaluation of PCR for diagnosis of visceral leishmaniasis. J Clin Microbiol. 1997; 35 (10): 2454-7. [PMID] [PMCID]
51. Sundar S. Diagnosis of kala-azar--an important stride. JAPI. 2003; 51: 753-5. [PMID]
52. Sundar S, Rai M. Laboratory diagnosis of visceral leishmaniasis. Clin Diagn Lab Immunol. 2002; 9 (5): 951-8. [DOI:10.1128/CDLI.9.5.951-958.2002]
53. Singh S, Sivakumar R. Recent advances in the diagnosis of leishmaniasis. J Postgrad Med. 2003; 49 (1): 55-60. [DOI:10.4103/0022-3859.927] [PMID]
54. Navin TR, Arana FE, de Merida AM, Arana BA, Castillo AL, Silvers DN. Cutaneous leishmaniasis in Guatemala: comparison of diagnostic methods. Am J Trop Med Hyg. 1990; 42 (1): 36-42. [DOI:10.4269/ajtmh.1990.42.36] [PMID]
55. Schubach A, Cuzzi-Maya T, Oliveira AV, Sartori A, de Oliveira-Neto MP, Mattos MS, et al. Leishmanial antigens in the diagnosis of active lesions and ancient scars of American tegumentary leishmaniasis patients. Mem Inst Oswaldo Cruz. 2001; 96 (7): 987-96. [DOI:10.1590/S0074-02762001000700018] [PMID]
56. Ohl CA, Hyams KC, Malone JD, Oldfield E 3rd. Leishmaniasis among Desert Storm veterans: a diagnostic and therapeutic dilemma. Mil Med. 1993; 158 (11): 726-9. [PMID]
57. Stockdale L, Newton R. A review of preventative methods against human leishmaniasis infection. PLoS Negl Trop Dis. 2013; 7 (6): e2278. [DOI:10.1371/journal.pntd.0002278] [PMID] [PMCID]
58. Khalil EA, Ayed NB, Musa AM, Ibrahim ME, Mukhtar MM, Zijlstra EE, et al. Dichotomy of protective cellular immune responses to human visceral leishmaniasis. Clin Exp Immunol. 2005; 140 (2): 349-53. [DOI:10.1111/j.1365-2249.2005.02768.x] [PMID] [PMCID]
59. Neogy AB, Nandy A, Chowdhury AB. Leishmanin test in post-kala-azar dermal leishmaniasis. Trans R Soc Trop Med Hyg. 1990; 84 (1): 58. [DOI:10.1016/0035-9203(90)90381-N]
60. Gidwani K, Rai M, Chakravarty J, Boelaert M, Sundar S. Evaluation of leishmanin skin test in Indian visceral leishmaniasis. Am J Trop Med Hyg. 2009; 80 (4): 566-7. [PMID]
61. Gadisa E, Custodio E, Canavate C, Sordo L, Abebe Z, Nieto J, et al. Usefulness of the rK39-immunochromatographic test, direct agglutination test, and leishmanin skin test for detecting asymptomatic Leishmania infection in children in a new visceral leishmaniasis focus in Amhara State, Ethiopia. Am J Trop Med Hyg. 2012; 86 (5): 792-8. [DOI:10.4269/ajtmh.2012.11-0196] [PMID] [PMCID]
62. Riera C, Fisa R, Lopez-Chejade P, Serra T, Girona E, Jimenez M, et al. Asymptomatic infection by Leishmania infantum in blood donors from the Balearic Islands (Spain). Transfusion. 2008; 48 (7): 1383-9. [DOI:10.1111/j.1537-2995.2008.01708.x] [PMID]
63. Zijlstra EE, Khalil EA, Kager PA, El-Hassan AM. Post-kala-azar dermal leishmaniasis in the Sudan: clinical presentation and differential diagnosis. Br J Dermatol. 2000; 143 (1): 136-43. [DOI:10.1046/j.1365-2133.2000.03603.x] [PMID]
64. Attar ZJ, Chance ML, el-Safi S, Carney J, Azazy A, El-Hadi M, et al. Latex agglutination test for the detection of urinary antigens in visceral leishmaniasis. Acta Trop. 2001; 78 (1): 11-6. [DOI:10.1016/S0001-706X(00)00155-8]
65. De Colmenares M, Portus M, Riera C, Gallego M, Aisa MJ, Torras S, et al. Short report: detection of 72-75-kD and 123-kD fractions of Leishmania antigen in urine of patients with visceral leishmaniasis. Am J Trop Med Hyg. 1995; 52 (5): 427-8. [DOI:10.4269/ajtmh.1995.52.427] [PMID]
66. Sarkari B, Chance M, Hommel M. Antigenuria in visceral leishmaniasis: detection and partial characterisation of a carbohydrate antigen. Acta Trop. 2002; 82 (3): 339-48. [DOI:10.1016/S0001-706X(02)00043-8]
67. Boelaert M, Rijal S, Regmi S, Singh R, Karki B, Jacquet D, et al. A comparative study of the effectiveness of diagnostic tests for visceral leishmaniasis. Am J Trop Med Hyg. 2004; 70 (1): 72-7. [PMID]
68. Kar K. Serodiagnosis of leishmaniasis. Crit Rev Microbiol. 1995; 21 (2): 123-52. [DOI:10.3109/10408419509113537] [PMID]
69. Braz RF, Nascimento ET, Martins DR, Wilson ME, Pearson RD, Reed SG, et al. The sensitivity and specificity of Leishmania chagasi recombinant K39 antigen in the diagnosis of American visceral leishmaniasis and in differentiating active from subclinical infection. Am J Trop Med Hyg. 2002; 67 (4): 344-8. [DOI:10.4269/ajtmh.2002.67.344] [PMID]
70. De Almeida Silva L, Romero HD, Prata A, Costa RT, Nascimento E, Carvalho SF, et al. Immunologic tests in patients after clinical cure of visceral leishmaniasis. Am J Trop Med Hyg. 2006; 75 (4): 739-43. [DOI:10.1111/j.1365-3156.2004.01212.x] [PMID]
71. Sundar S, Singh RK, Maurya R, Kumar B, Chhabra A, Singh V, et al. Serological diagnosis of Indian visceral leishmaniasis: direct agglutination test versus rK39 strip test. Trans R Soc Trop Med Hyg. 2006; 100 (6): 533-7. [DOI:10.1016/j.trstmh.2005.08.018] [PMID]
72. Iqbal J, Hira PR, Saroj G, Philip R, Al-Ali F, Madda PJ, et al. Imported visceral leishmaniasis: diagnostic dilemmas and comparative analysis of three assays. J Clin Microbiol. 2002; 40 (2): 475-9. [DOI:10.1128/JCM.40.3.475-479.2001] [PMID] [PMCID]
73. Badaro R, Reed SG, Carvalho EM. Immunofluorescent antibody test in American visceral leishmaniasis: sensitivity and specificity of different morphological forms of two Leishmania species. Am J Trop Med Hyg. 1983; 32 (3): 480-4. [DOI:10.4269/ajtmh.1983.32.480] [PMID]
74. Kumar R, Pai K, Pathak K, Sundar S. Enzyme-linked immunosorbent assay for recombinant K39 antigen in diagnosis and prognosis of Indian visceral leishmaniasis. Clin Diagn Lab Immunol. 2001; 8 (6): 1220-4. [DOI:10.1128/CDLI.8.6.1220-1224.2001]
75. Houghton RL, Petrescu M, Benson DR, Skeiky YA, Scalone A, Badaro R, et al. A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type 1 patients and a prognostic indicator for monitoring patients undergoing drug therapy. J Infect Dis. 1998; 177 (5): 1339-44. [DOI:10.1086/515289] [PMID]
76. Pedras MJ, Orsini M, Castro M, Passos VM, Rabello A. Antibody subclass profile against Leishmania braziliensis and Leishmania amazonensis in the diagnosis and follow-up of mucosal leishmaniasis. Diagn Microbiol Infect Dis. 2003; 47 (3): 477-85. [DOI:10.1016/S0732-8893(03)00141-X]
77. Zijlstra EE, Daifalla NS, Kager PA, Khalil EA, El-Hassan AM, Reed SG, et al. rK39 enzyme-linked immunosorbent assay for diagnosis of Leishmania donovani infection. Clin Diagn Lab Immunol. 1998; 5 (5): 717-20. [PMID] [PMCID]
78. Srivastava P, Dayama A, Mehrotra S, Sundar S. Diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg. 2011; 105 (1): 1-6. [DOI:10.1016/j.trstmh.2010.09.006] [PMID] [PMCID]
79. Sivakumar R, Sharma P, Chang KP, Singh S. Cloning, expression, and purification of a novel recombinant antigen from Leishmania donovani. Protein Expr Purif. 2006; 46 (1): 156-65. [DOI:10.1016/j.pep.2005.07.027] [PMID]
80. Pattabhi S, Whittle J, Mohamath R, El-Safi S, Moulton GG, Guderian JA, et al. Design, development and evaluation of rK28-based point-of-care tests for improving rapid diagnosis of visceral leishmaniasis. PLoS Negl Trop Dis. 2010; 4 (9): pii: e822. [DOI:10.1371/journal.pntd.0000822] [PMID] [PMCID]
81. Abdallah KA, Nour BY, Schallig HD, Mergani A, Hamid Z, Elkarim AA, et al. Evaluation of the direct agglutination test based on freeze-dried Leishmania donovani promastigotes for the serodiagnosis of visceral leishmaniasis in Sudanese patients. Trop Med Int Health. 2004; 9 (10): 1127-31. [DOI:10.1111/j.1365-3156.2004.01308.x] [PMID]
82. Fakhar M, Motazedian MH, Hatam GR, Asgari Q, Monabati A, Keighobadi M. Comparative performance of direct agglutination test, indirect immunofluorescent antibody test, polymerase chain reaction and bone marrow aspiration method for diagnosis of Mediterranean visceral leishmaniasis. Afr J Microbiol Res. 2012; 6 (28): 5777-81.
83. Boelaert M, El Safi S, Mousa H, Githure J, Mbati P, Gurubacharya V, et al. Multi-centre evaluation of repeatability and reproducibility of the direct agglutination test for visceral leishmaniasis. Trop Med Int Health. 1999; 4 (1): 31-7. [DOI:10.1046/j.1365-3156.1999.00348.x] [PMID]
84. Akhoundi B, Mohebali M, Babakhan L, Edrissian GH, Eslami MB, Keshavarz H, et al. Rapid detection of human Leishmania infantum infection: a comparative field study using the fast agglutination screening test and the direct agglutination test. Travel Med Infect Dis. 2010; 8 (5): 305-10. [DOI:10.1016/j.tmaid.2010.09.001] [PMID]
85. Islam MZ, Itoh M, Mirza R, Ahmed I, Ekram AR, Sarder AH, et al. Direct agglutination test with urine samples for the diagnosis of visceral leishmaniasis. Am J Trop Med Hyg. 2004; 70 (1): 78-82. [PMID]
86. Rezvan H. Evaluation of Different Approaches in Leishmania Diagnosis. Int J Adv Biol Biom Res. 2014; 2 (2): 238-61.
87. Ravindran R, Anam K, Bairagi BC, Saha B, Pramanik N, Guha SK, et al. Characterization of immunoglobulin G and its subclass response to Indian kala-azar infection before and after chemotherapy. Infect Immun. 2004; 72 (2): 863-70. [DOI:10.1128/IAI.72.2.863-870.2004] [PMID] [PMCID]
88. Kumar P, Pai K, Tripathi K, Pandey HP, Sundar S. Immunoblot analysis of the humoral immune response to Leishmania donovani polypeptides in cases of human visceral leishmaniasis: its usefulness in prognosis. Clin Diagn Lab Immunol. 2002; 9 (5): 1119-23. [DOI:10.1128/CDLI.9.5.1119-1123.2002]
89. Mandal J, Khurana S, Dubey ML, Bhatia P, Varma N, Malla N. Evaluation of direct agglutination test, rk39 Test, and ELISA for the diagnosis of visceral leishmaniasis. Am J Trop Med Hyg. 2008; 79 (1): 76-8. [PMID]
90. Gao CH, Yang YT, Shi F, Wang JY, Steverding D, Wang X. Development of an Immunochromatographic Test for Diagnosis of Visceral Leishmaniasis Based on Detection of a Circulating Antigen. PLoS Negle Trop Dis. 2015; 9 (6): e0003902. [DOI:10.1371/journal.pntd.0003902] [PMID] [PMCID]
91. Carvalho SF, Lemos EM, Corey R, Dietze R. Performance of recombinant K39 antigen in the diagnosis of Brazilian visceral leishmaniasis. Am J Trop Med Hyg. 2003; 68 (3): 321-4. [PMID]
92. Brandonisio O, Fumarola L, Maggi P, Cavaliere R, Spinelli R, Pastore G. Evaluation of a rapid immunochromatographic test for serodiagnosis of visceral leishmaniasis. Eur J Clin Microbiol Infect Dis. 2002; 21 (6): 461-4. [DOI:10.1007/s10096-002-0739-8] [PMID]
93. Sundar S, Pai K, Sahu M, Kumar V, Murray HW. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol. 2002; 96 (1): 19-23. [DOI:10.1179/000349802125000466] [PMID]
94. Jelinek T, Eichenlaub S, Loscher T. Sensitivity and specificity of a rapid immunochromatographic test for diagnosis of visceral leishmaniasis. Eur J Clin Microbiol Infect Dis. 1999; 18 (9): 669-70. [DOI:10.1007/s100960050371] [PMID]
95. Zijlstra EE, Nur Y, Desjeux P, Khalil EA, El-Hassan AM, Groen J. Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan. Trop Infect Int Healt. 2001; 6 (2): 108-13. [DOI:10.1046/j.1365-3156.2001.00680.x]
96. Elmahallawy EK, Martinez AS, Rodriguez-Granger J, Hoyos-Mallecot Y, Agil A, Mari JMN, et al. Diagnosis of leishmaniasis. J Infect Dev Ctries. 2014; 8 (08): 961-72. [DOI:10.3855/jidc.4310] [PMID]
97. Silveira FT, Lainson R, De Souza AA, Campos MB, Carneiro LA, Lima LV, et al. Further evidences on a new diagnostic approach for monitoring human Leishmania (L.) infantum chagasi infection in Amazonian Brazil. Parasitol Res. 2010; 106 (2): 377-86. [DOI:10.1007/s00436-009-1672-x] [PMID]
98. Deborggraeve S, Laurent T, Espinosa D, Van der Auwera G, Mbuchi M, Wasunna M, et al. A simplified and standardized polymerase chain reaction format for the diagnosis of leishmaniasis. J Infect Dis. 2008; 198 (10): 1565-72. [DOI:10.1086/592509] [PMID]
99. Srivastava P, Mehrotra S, Tiwary P, Chakravarty J, Sundar S. Diagnosis of Indian visceral leishmaniasis by nucleic acid detection using PCR. PloS one. 2011; 6 (4): e19304. [DOI:10.1371/journal.pone.0019304] [PMID] [PMCID]
100. Dey A, Singh S. Genetic heterogeneity among visceral and post-Kala-Azar dermal leishmaniasis strains from eastern India. Infect Genet Evol. 2007; 7 (2): 219-22. [DOI:10.1016/j.meegid.2006.09.001] [PMID]
101. Mauricio IL, Stothard JR, Miles MA. Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon. Parasitol. 2004; 128 (Pt 3): 263-7. [DOI:10.1017/S0031182003004578]
102. Salotra P, Sreenivas G, Beena KR, Mukherjee A, Ramesh V. Parasite detection in patients with post kala-azar dermal leishmaniasis in India: a comparison between molecular and immunological methods. J Clin Pathol. 2003; 56 (11): 840-3. [DOI:10.1136/jcp.56.11.840] [PMID] [PMCID]
103. De Doncker S, Hutse V, Abdellati S, Rijal S, Singh Karki BM, Decuypere S, et al. A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects. Trans R Soc Trop Med Hyg. 2005; 99 (1): 25-31. [DOI:10.1016/j.trstmh.2004.01.015] [PMID]
104. le Fichoux Y, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, et al. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J Clin Microbiol. 1999; 37 (6): 1953-7. [PMID] [PMCID]
105. Wortmann G, Sweeney C, Houng HS, Aronson N, Stiteler J, Jackson J, et al. Rapid diagnosis of leishmaniasis by fluorogenic polymerase chain reaction. Am J Trop Med Hyg. 2001; 65 (5): 583-7. [DOI:10.4269/ajtmh.2001.65.583] [PMID]
106. Mary C, Faraut F, Lascombe L, Dumon H. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol. 2004; 42 (11): 5249-55. [DOI:10.1128/JCM.42.11.5249-5255.2004] [PMID] [PMCID]
107. Shafiei R, Mohebali M, Akhoundi B, Galian MS, Kalantar F, Ashkan S, et al. Emergence of co-infection of visceral leishmaniasis in HIV-positive patients in northeast Iran: a preliminary study. Travel Med Infect Dis. 2014; 12 (2): 173-8. [DOI:10.1016/j.tmaid.2013.09.001] [PMID]
108. Albrecht H. Leishmaniosis--new perspectives on an underappreciated opportunistic infection. AIDS (London, England). 1998; 12 (16): 2225-6. [DOI:10.1097/00002030-199816000-00019]
109. Desjeux P, Alvar J. Leishmania/HIV co-infections: epidemiology in Europe. Ann Trop Med Parasitol. 2003; 97 Suppl 1: 3-15. [DOI:10.1179/000349803225002499] [PMID]
110. Chaara D, Haouas N, Dedet JP, Babba H, Pratlong F. Leishmaniases in Maghreb: an endemic neglected disease. Acta Trop. 2014; 132: 80-93. [DOI:10.1016/j.actatropica.2013.12.018] [PMID]
111. Azmi K, Schonian G, Schnur LF, Nasereddin A, Ereqat S, Abdeen Z. Development of assays using hexokinase and phosphoglucomutase gene sequences that distinguish strains of Leishmania tropica from different zymodemes and microsatellite clusters and their application to Palestinian foci of cutaneous leishmaniasis. PLoS Negl Trop Dis. 2013; 7 (9): e2464. [DOI:10.1371/journal.pntd.0002464] [PMID] [PMCID]
112. Rodriguez N, Guzman B, Rodas A, Takiff H, Bloom BR, Convit J. Diagnosis of cutaneous leishmaniasis and species discrimination of parasites by PCR and hybridization. J Clin Microbiol. 1994; 32 (9): 2246-52. [PMID] [PMCID]
113. Montalvo AM, Fraga J, El Safi S, Gramiccia M, Jaffe CL, Dujardin JC, et al. Direct Leishmania species typing in Old World clinical samples: evaluation of 3 sensitive methods based on the heat-shock protein 70 gene. Diagn Microbiol Infect Dis. 2014; 80 (1): 35-9. [DOI:10.1016/j.diagmicrobio.2014.05.012] [PMID]
114. Behravan M, Moin-Vaziri V, Haghighi A, Rahbarian N, Taghipour N, Abadi A, et al. Molecular Identification of Leishmania Species in a Re-Emerged Focus of Cutaneous Leishmaniasis in Varamin District, Iran. J Arthropod Borne Dis. 2017; 11 (1): 124-31.
115. Shirmohammadi H, Kayedi MH, Reza M, Abai AZR, Moradi M, Mohebali M, et al. First molecular detection of Leishmania major DNA within Meriones persicus in new focus of cutaneous leishmaniasis in Lorestan Province, West of Iran. J Entomol Zool Stud. 2017; 5 (2): 1187-90.
116. Rezai A, Moghaddas E, Bagherpor MR, Naseri A, Shamsian SA. Identification of Leishmania Species for Cutaneous leishmaniasis in Gonabad, Bardaskan and Kashmar, Central Khorasan, 2015. Jundishapur J Microbiol. 2017; 10 (4): e44469. [DOI:10.5812/jjm.44469]
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Rezvan H, Nourian A R, Hamoon Navard S. An Overview on Leishmania Diagnosis. JoMMID . 2017; 5 (1 and 2) :1-11
URL: http://jommid.pasteur.ac.ir/article-1-118-en.html


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